毕超; 王焕丽; 梁景耀; 梁艳华; 朱慧兰; 王建琴

doi:10.3760/cma.j.issn.1007-1245.2020.17.008

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• 科研课题 •

女性尿液沙眼衣原体DNA和淋病奈瑟菌DNA定性检测结果分析

国际医药卫生导报, 2020,26(17) : 2523-2526. DOI: 10.3760/cma.j.issn.1007-1245.2020.17.008

摘要

目的

探讨用女性尿液检测沙眼衣原体（chlamydia trachomatis，CT）DNA和淋病奈瑟菌（neisseria gonorrhoeae，NG）DNA的临床意义。

方法

随机选取2019年1—6月性病科女性患者212例，每位患者取宫颈拭子做CT-DNA、NG-DNA、淋球菌培养，尿液做CT-DNA、NG-DNA。同时设置对照组30例。

结果

宫颈拭子CT-DNA阳性率为18.40%（39/212）；尿液CT-DNA阳性率为19.81%（42/212），二者阳性率比较差异无统计学意义（χ²=0.14，P=0.71）；宫颈拭子CT-DNA和尿液CT-DNA检测结果一致性好（Kappa=0.92，P<0.01）。淋球菌培养阳性率为5.19%（11/212），宫颈拭子NG-DNA阳性率为12.26%（26/212），二者阳性率比较差异有统计学意义（χ²=6.662，P=0.010）；尿液NG-DNA阳性率为10.38%（22/212），与淋球菌培养阳性率相比，差异有统计学意义（χ²=3.976，P=0.046）；宫颈拭子NG-DNA阳性率与尿液NG-DNA阳性率相比，差异无统计学意义（χ²=0.376，P=0.540）。宫颈拭子NG-DNA与尿液NG-DNA检测结果一致性好（Kappa=0.86，P<0.01）。

结论

用女性尿液检测CT-DNA、NG-DNA敏感性高、特异性好，取材方便，建议在临床普及。

引用本文: 毕超, 王焕丽, 梁景耀, 等. 女性尿液沙眼衣原体DNA和淋病奈瑟菌DNA定性检测结果分析 [J] . 国际医药卫生导报, 2020, 26(17) : 2523-2526. DOI: 10.3760/cma.j.issn.1007-1245.2020.17.008.

参考文献导出: Endnote NoteExpress RefWorks NoteFirst 医学文献王

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生殖道沙眼衣原体（chlamydia trachomatis，CT）感染和淋病是两种常见的性传播疾病，也是我国法定报告疫情的性病。近几年，我国生殖道CT感染和淋病发病率居高不下。2018年我国淋病报告发病数位居法定报告乙类传染病的第4位^[1]。生殖道CT感染自2008—2015年以每年2%的比例持续增长，发病率高于全国梅毒和淋病报告发病率^[2]。这两种性病都有无症状感染者，约有70%的女性沙眼衣原体感染后无任何临床表现^[3,4,5]，60%的女性淋病患者无明显临床经过，如果不及时治疗，可引起宫颈炎、不孕、不育、异位妊娠、输卵管堵塞和早产、盆腔炎等一系列严重后果^[6,7]。因此，为了实行早期治疗，预防危害的发生，采用高敏感性和特异性的检测方法及时发现感染者至关重要。应用PCR原理检测尿液CT-DNA和淋球菌DNA是近年来在临床应用的新方法，该方法采用无创性标本，并可全自动提取样本核酸进行扩增反应，同时检测CT-DNA和淋病奈瑟菌（neisseria gonorrhoeae，NG）-DNA，适合大批量样本筛查。本研究通过该方法对性病门诊女性患者尿液CT-DNA和NG-DNA进行筛查，现报道如下。

贡献者信息

毕超

广州市皮肤病防治所检验科　510095

王焕丽

广州市皮肤病防治所检验科　510095

梁景耀

广州市皮肤病防治所检验科　510095

梁艳华

广州市皮肤病防治所检验科　510095

朱慧兰

广州市皮肤病防治所检验科　510095

王建琴

广州市皮肤病防治所检验科　510095

通信作者

王建琴

广州市皮肤病防治所检验科　510095

Email：jianqinwang@foxmail.com

关键词

尿液; 沙眼衣原体-DNA; 淋球菌-DNA;

基金项目

广州市卫生计生科技西医类重大项目（20181A031003）

利益冲突

利益冲突：

作者已申明文章无相关利益冲突。

历史

出版日期：2020-09-01

收稿日期：2020-03-26

本文责任校对

刘玲玲

Scientific Research

Quantitative analysis of DNA of Chlamydia trachomatis and Neisseria gonorrhoeae of female urine

Bi Chao, Wang Huanli, Liang Jingyao, Liang Yanhua, Zhu Huilan, Wang Jianqin

Published 2020-09-01

Cite as IMHGN, 2020, 26(17): 2523-2526. DOI: 10.3760/cma.j.issn.1007-1245.2020.17.008

Abstract

Objective

To investigate the clinical significance of detecting Chlamydia trachomatis (CT) DNA and Neisseria gonorrhoeae (NG) DNA in female urine.

Methods

From January to June 2019, 212 female patients in department of venereal disease, were randomly selected. Cervical swabs were taken from each patient to detect CT-DNA, NG-DNA, Neisseria gonorrhoeae culture, and urine was used to detect CT-DNA, NG-DNA. Thirty female examinees were chosen as the control group.

Results

CT-DNA positive rate of cervical swabs was 18.40% (39/212); CT-DNA positive rate of urine was 19.81% (42/212); there was no statistically significant difference between the positive rates of the two (χ²=0.14, P=0.71). The results of cervical swab CT-DNA and urine CT-DNA were consistent (Kappa=0.92, P<0.01). The positive rate of N. gonorrhoeae culture was 5.19% (11/212), and the positive rate of NG-DNA in cervical swabs was 12.26% (26/212), there was statistically significant difference between the positive rates of the two (χ²=6.662, P=0.010); the NG-DNA positive rate of urine was 10.38% (22/212), there was statistically significant difference compared with the positive rate of N. gonorrhoeae culture (χ²=3.976, P=0.046); the NG-DNA positive rate of cervical swabs was similar to that of urine (χ²=0.376, P=0.540). The results of cervical swab NG-DNA and urine NG-DNA were consistent (Kappa=0.86, P<0.01).

Conclusion

Urine is a non-invasive specimen. The detection of CT-DNA and NG-DNA of urine in female patients has high sensitivity and specificity, suggested for clinical popularity.

Key words:

Urine; Chlamydia trachomatis (CT) DNA; Neisseria gonorrhoeae (NG) DNA

Contributor Information

Bi Chao