Original Article
Influence of CCL21 on the invasion and metastasis of colorectal cancer
Rong Yuming, Chen Xi, Fan Dejun, Lin Xutao, Zheng Xiaobin, Zhou Chi, Hu Tuo, Zou Yifeng
Published 2017-11-25
Cite as Chin J Gastrointest Surg, 2017, 20(11): 1300-1305. DOI: 10.3760/cma.j.issn.1671-0274.2017.11.018
Abstract
ObjectiveTo investigate the influence of CCL21 on the invasion and metastasis of colorectal cancer (CRC) .
MethodsCCL21 over-expressing CRC cell line was constructed by lentivirus infection and CCL21 low-expressing CRC cell line was constructed by lipofection. The effects of CCL21 on the invasion and metastasis of CRC cells and the stem cell-like phenotype were investigated by Transwell migration, invasion assay, wound healing assay and sphere formation assay.
ResultsReal-time quantitative PCR and western blot confirmed that the expression of CCL21 was up-regulated by lentiviral transfection and down-regulated by siRNA liposome transfection. In vitro, Transwell assays showed that the invasion and migration in CCL21 over-expressing CRC cells decreased significantly as compared to those of CCL21 low-expressing cells. In wound healing assay, the CCL21 over-expressing CRC cells showed a significantly lower rate of migration. In addition, the sphere formation rate and density of CCL21 over-expressing CRC cells were lower than those with low-expression of CCL21.
ConclusionCCL21 can suppress the migration and invasion of CRC cells and weaken their stem cell-like phenotype.
Key words:
Colorectal neoplasms; CCL21; Invasion and metastasis; Stem cell-like phenotype
Contributor Information
Rong Yuming
VIP Region, Sun Yat-sen University Cancer Center, Guangzhou 510060, China, The Sixth Affiliated Hospital, Sun Yat-sen University, Guangzhou 510655, China
Chen Xi
Department of Colorectal Surgery, The Sixth Affiliated Hospital, Sun Yat-sen University, Guangzhou 510655, China
Fan Dejun
Department of Gastrointestinal Endoscopy, The Sixth Affiliated Hospital, Sun Yat-sen University, Guangzhou 510655, China
Lin Xutao
Department of Gastrointestinal Endoscopy, The Sixth Affiliated Hospital, Sun Yat-sen University, Guangzhou 510655, China
Zheng Xiaobin
Department of Colorectal Surgery, The Sixth Affiliated Hospital, Sun Yat-sen University, Guangzhou 510655, China
Zhou Chi
Department of Colorectal Surgery, The Sixth Affiliated Hospital, Sun Yat-sen University, Guangzhou 510655, China
Hu Tuo
Department of Colorectal Surgery, The Sixth Affiliated Hospital, Sun Yat-sen University, Guangzhou 510655, China
Zou Yifeng
Department of Colorectal Surgery, The Sixth Affiliated Hospital, Sun Yat-sen University, Guangzhou 510655, China
