Objective To investigate the human mitochondrial DNA (mtDNA) variations associated with longevity in Bama elderly population from Guangxi. Methods Mitochondrial genome of 20 individuals over 96 years of age was sequenced, and seven target single nucleotide polymorphism(SNPs) were observed by comparing with the standard rCRS sequence, and two were tested by polymerase chain reactionrestriction fragment length polymorphism (PCR-RFLP) method in a larger population including 208 individuals of 90-113 years old, and 586 unrelated control individuals from Guangxi. Results The 4824G frequency of the mtDNA4824A/G locus increased with age both in the long-lived elderly and in controls.And it was significantly higher in controls than that in long-lived population (P＜0.05). Conclusion The mtDNA4824 A/G is not only an age-related locus, its mutation is also negatively correlated with longevity.