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ENGLISH ABSTRACT
双醋瑞因对眼表常见病原菌的体外抑制作用
岳娟
简守珺
吕奇学
孙声桃
韩雷
刘素素
张红敏
作者及单位信息
·
DOI： 10.3760/cma.j.issn.2095-0160.2020.03.002
In vitroantimicrobial activity of diacerein on common ocular surface pathogens
Yue Juan
Jian Shoujun
Lyu Qixue
Sun Shengtao
Han Lei
Liu Susu
Zhang Hongmin
Authors Info & Affiliations
Yue Juan
Department of Ophthalmology, Henan Provincial People's Hospital, Henan Eye Hospital, Henan Eye Institute, Henan Key Laboratory of Ophthalmology and Visual Science, Zhengzhou University People's eye Hospital, Zhengzhou 450003, China
Jian Shoujun
Lyu Qixue
Sun Shengtao
Han Lei
Liu Susu
Zhang Hongmin
·
DOI： 10.3760/cma.j.issn.2095-0160.2020.03.002
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摘要

目的研究双醋瑞因对眼表常见病原菌的体外抑菌作用。

方法采集于河南省立眼科医院就诊的眼表感染患者中分离培养的革兰阳性球菌和杆菌、革兰阴性杆菌、丝状真菌及念珠菌。采用K-B琼脂纸片扩散法测定双醋瑞因对病原菌的体外抑菌活性，采用微量液基稀释法进行最低抑菌浓度(MIC)定量测定。以左氧氟沙星和伏立康唑分别为抗细菌药物和抗真菌药物对照。

结果双醋瑞因对眼表分离的42株革兰阳性球菌和10株革兰阳性杆菌均有明显抑菌活性，双醋瑞因与左氧氟沙星对表皮葡萄球菌、金黄色葡萄球菌、中间葡萄球菌和革兰阳性棒状杆菌抑菌圈直径比较，差异无统计学意义(均P>0.05)。双醋瑞因对眼表分离的23株革兰阴性杆菌，10株丝状真菌和3株念珠菌均无抑制作用。双醋瑞因对表皮葡萄球菌、金黄色葡萄球菌、中间葡萄球菌及其他葡萄球菌的MIC范围为1～32 μg/ml，MIC 90分别为16、8、16、32 μg/ml。

结论双醋瑞因对眼表分离的革兰阳性葡萄球菌和棒状杆菌具有体外抑菌活性，对致病革兰阴性杆菌和真菌无抑菌活性。

革兰阳性球菌;杆菌;丝状真菌;念珠菌;双醋瑞因;抑菌圈;K-B琼脂纸片扩散法
ABSTRACT

ObjectiveTo study the antimicrobial activity of diacerein on common pathogens of the ocular surface in vitro.

MethodsPathogens were collected from patients with ocular surface infections in Henan Eye Hospital, including Gram-positive cocci and bacilli, Gram-negative bacilli, filamentous fungi, and Candida.The antimicrobial activity of diacerein was determined by the K-B agar diffusion method, and its minimum inhibitory concentration (MIC) was determined by the micro-liquid method.Levofloxacin and voriconazole were used as the control of antibacterial and antifungal drug, respectirely.

ResultsDiacerein showed antibacterial activity against 42 strains of Gram-positive cocci and 10 strains of Gram-positive bacilli, its inhibition zone diameters for Staphylococcus epidermidis, S.aureus, S.intermedius and Gram-positive Corynebacterium were not significantly different from those of levofloxacin (all at P>0.05). Its MIC range of diacetate against Staphylococcus epidermidis, S. aureus, S. intermedius and other Staphylococci was 1-32 μg/ml, and its respective MIC 90 was 16, 8, 16, and 32 μg/ml.Diacerein had no bacteriostatic effect on 23 strains of Gram-negative bacilli, 10 strains of filamentous fungi and 3 strains of candida.

ConclusionsDiacerein has antibacterial effects against Gram-positive Staphylococcus and Corynebacterium isolated from the ocular surface, but shows no antimicrobial activity against Gram-negative bacilli and fungi.Diacerein offers a new drug option and method for the treatment of bacterial keratitis.

Gram-positive cocci;Bacilli;Filamentous fungi;Candida;Diacerein;Inhibition zone;K-B agar paper diffusion method
Zhang Hongmin, Email: mocdef.3ab616090mhz
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引用本文

岳娟,简守珺,吕奇学,等. 双醋瑞因对眼表常见病原菌的体外抑制作用[J]. 中华实验眼科杂志,2020,38(03)：165-168.

DOI:10.3760/cma.j.issn.2095-0160.2020.03.002

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细菌和真菌是眼表常见的病原微生物。抗菌药物的不规范使用导致部分病原菌产生耐药性；由于角膜屏障的存在，有些在全身感染性疾病治疗中有效的药物在眼部用药时通透性不佳，在病变部分难以达到有效药物浓度，影响药物疗效，筛选眼部新型抑菌药物一直是临床研究的热点和难点 [ 1 , 2 ]。双醋瑞因是白细胞介素-1抑制剂，主要用于治疗骨关节炎 [ 3 ]，但迄今为止该药尚未应用于眼科疾病的治疗 [ 4 , 5 ]。本研究团队前期制备了双醋瑞因滴眼液并进行角膜的药代动力学和抗炎药效学研究，结果显示该药可以在感染性角膜炎的早期抑制病程的发展，并具有良好的角膜穿透性 [ 6 , 7 ]。研究显示，多数蒽醌类化合物具有抑菌活性 [ 8 ]。双醋瑞因为蒽醌类小分子化合物，其是否具有抑菌作用，目前仍不十分清楚。本研究探讨双醋瑞因滴眼液对眼表常见致病球菌、杆菌、丝状真菌及念珠菌的体外抑菌作用。
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备注信息
A
张红敏，Email： mocdef.3ab616090mhz
B
所有作者声明不存在利益冲突
C
国家自然科学基金项目 (81770902)
河南省自然科学基金项目 (162300410163)
河南省科技创新人才计划项目 (144100510015)
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