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ENGLISH ABSTRACT
流式细胞术在眼部疾病细胞因子和细胞学检查中的应用进展
刘铭
陶勇 [综述]
作者及单位信息
·
DOI： 10.3760/cma.j.cn115989-20210118-00049
Application progress of flow cytometry in the cytokine test and cytological examination for ocular diseases
Liu Ming
Tao Yong
Authors Info & Affiliations
Liu Ming
Beijing GiantMed Medical Diagnostics Laboratory, Beijing 101399, China
Tao Yong
Beijing Chaoyang Hospital, Capital Medical University, Beijing 100020, China
·
DOI： 10.3760/cma.j.cn115989-20210118-00049
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摘要

流式细胞术是一种能够对单个细胞或生物颗粒进行多参数、定量分析和分选的检测技术，是近年来迅速发展起来的一项先进的细胞定量分析技术，广泛应用于免疫学、肿瘤学和血液学等多领域医学研究和临床诊断。该技术可以定量检测和分析眼内液中细胞因子水平及各细胞亚群的分布特征。与传统的酶联免疫吸附试验、细胞形态学检查及免疫组织化学检测等方法比较，流式细胞术具有操作简单、所需样本量小、灵敏度高、通量高等优点。流式细胞术已广泛应用于眼内新生血管相关疾病、眼内淋巴瘤、视网膜病变、白内障、葡萄膜炎、结节病性葡萄膜炎、感染性眼内炎症性疾病等眼部疾病相关细胞因子水平检测和细胞亚群分析中，对眼部疾病的发病机制研究和靶向治疗起到日益重要的作用。本文就流式细胞术在眼部疾病眼内液的细胞因子和细胞学检查中的应用进展进行综述。

流式细胞术;眼部疾病;眼内液;细胞因子;细胞亚群
ABSTRACT

As an advanced quantitative technology for cells, flow cytometry, which can make multi-paramter quantitative analysis and sorting of individual cells or biological particles, has been widely used in immunology, oncology, hematology and other fields of medical research and clinical diagnosis in recent years.It can quantitatively detect the expression levels of cytokines and the distribution characteristics of cell subsets in the intraocular fluid.Compared with traditional enzyme-linked immunosorbent assay, cell morphology and immunohistochemistry, it has the advantages of simple operation, smaller sample size, higher sensitivity and higher throughput.Flow cytometry has been widely used in the detection and subgroup analysis of cytokines related to eye diseases, such as intraocular angiogenesis, intraocular lymphoma, retinopathy, cataract, uveitis, sarcoidotic uveitis, infectious intraocular inflammation, etc.Flow cytometry plays an increasingly important role in the pathogenesis research and targeted therapy of eye diseases.In this article, the application of flow cytometry in the examination of cytokines and cytology in ocular fluid for ocular diseases were reviewed.

Flow cytometry;Eye diseases;Intraocular fluid;Cytokine;Cell subsets
Tao Yong, Email: mocdef.habycjbgnoyoat
Shunyi District "Beijing Science and Technology Achievements Transformation Coordination and Service Platform" Construction Fund
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引用本文

刘铭,陶勇. 流式细胞术在眼部疾病细胞因子和细胞学检查中的应用进展[J]. 中华实验眼科杂志,2021,39(03)：269-272.

DOI:10.3760/cma.j.cn115989-20210118-00049

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流式细胞术是一种能够对单个细胞或生物颗粒进行多参数、定量分析和分选的检测技术，具有速度快、精度高、准确性好和通量高等优点，是目前先进的细胞定量分析技术之一，广泛应用于免疫学、肿瘤学、血液学等领域的医学研究和临床诊断，特别是免疫学的淋巴细胞亚群分析。淋巴细胞亚群分析是临床开展最多的检测项目，也是检测机体免疫功能的重要指标，对疾病的诊断、发病机制研究、疾病的治疗和预后具有重要意义 [ 1 , 2 , 3 ]。随着流式细胞术的发展以及眼科基础医学研究和临床精准诊疗要求的不断提高，流式细胞术越来越多地用于眼部疾病的研究与辅助靶向治疗，该技术可以定量检测眼内液中细胞因子水平、细胞亚群的占比，对研究眼部疾病的发病机制和分类诊疗具有重要意义。本文就流式细胞术在眼部疾病眼内液中细胞因子和细胞学检查的应用进展综述。
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备注信息
A
陶勇，Email： mocdef.habycjbgnoyoat
B
刘铭为北京智德医学检验所有限公司职员，陶勇声明不存在任何利益冲突
C
国家自然科学基金项目 (82070948)
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