高级检索
中华生物医学工程杂志
期刊首页
过刊列表
高级检索
稿件发表
• 论著 •
ENGLISH ABSTRACT
四氟化钛溶液对酸蚀症牙本质粘接强度的影响
孙傲
马欣蓉
刘晓强
谭建国
作者及单位信息
·
DOI： 10.3760/cma.j.cn115668-20200201-00019
Effect of titanium tetrafluoride solution on bonding strength of acid-etched dentin
Sun Ao
Ma Xinrong
Liu Xiaoqiang
Tan Jianguo
Authors Info & Affiliations
Sun Ao
Department of Prosthodontics, Peking University Hospital of Stomatology, National Clinical Research Center for Oral Diseases, National Engineering Laboratory for Digital and Material Technology of Stomatology, Beijing Key Laboratory of Digital Stomatology, Beijing 100081, China
Ma Xinrong
Department of Prosthodontics, Peking University Hospital of Stomatology, National Clinical Research Center for Oral Diseases, National Engineering Laboratory for Digital and Material Technology of Stomatology, Beijing Key Laboratory of Digital Stomatology, Beijing 100081, China
Liu Xiaoqiang
Department of Prosthodontics, Peking University Hospital of Stomatology, National Clinical Research Center for Oral Diseases, National Engineering Laboratory for Digital and Material Technology of Stomatology, Beijing Key Laboratory of Digital Stomatology, Beijing 100081, China
Tan Jianguo
Department of Prosthodontics, Peking University Hospital of Stomatology, National Clinical Research Center for Oral Diseases, National Engineering Laboratory for Digital and Material Technology of Stomatology, Beijing Key Laboratory of Digital Stomatology, Beijing 100081, China
·
DOI： 10.3760/cma.j.cn115668-20200201-00019
228
17
0
0
0
0
PDF下载
APP内阅读
摘要

目的研究四氟化钛（TiF 4）溶液对酸蚀症牙本质粘接强度的影响。

方法选取24颗新鲜拔除的人第三磨牙，获取冠部近髓腔的牙本质，随机分为6组，每组4颗牙，其中4组通过酸蚀和机械力方法建立人工酸蚀症牙本质模型，另外2组为健康牙本质。随机选取1组人工酸蚀症牙本质和1组健康牙本质，使用扫描电镜和显微硬度仪测定其表面形貌与表面硬度，评价人工酸蚀症牙本质的建模效果。另外3组人工酸蚀症牙本质和1组健康牙本质用于粘接测试，按不同处理分为4组：4.0% TiF 4处理酸蚀症牙本质组（4.0% TiF 4-ED组）、2.5% TiF 4处理酸蚀症牙本质组（2.5% TiF 4-ED组）、无处理酸蚀症牙本质组（ED组）及健康牙本质组（SD组）。使用SE Bond自酸蚀粘接系统进行粘接，AP-X复合树脂分层充填，测定即刻微拉伸粘接强度并观察断裂模式。

结果人工酸蚀症牙本质和健康牙本质的显微硬度值分别为（56.92±5.27）HV和（60.19±4.52）HV，组间比较差异有统计学意义（ P<0.05）。扫描电镜结果证实，人工酸蚀症牙本质与自然酸蚀症牙本质的微观形貌特征相符。4.0% TiF 4-ED组、2.5% TiF 4-ED组、ED组和SD组的微拉伸粘接强度分别为（28.94±8.04）MPa、（46.08±8.19）MPa、（20.39±7.46）MPa和（45.16±7.86）MPa。2.5% TiF 4-ED组和SD组的微拉伸粘接强度比较，差异无统计学意义（ P=0.529），二者均高于4.0% TiF 4-ED组和ED组（均 P<0.05）。4组断裂模式分布比较，差异有统计学意义（ χ 2=54.91， P<0.001）。2.5% TiF 4-ED组以内聚牙本质断裂为主，提示其粘接强度可能高于微拉伸测试均值。

结论酸蚀症牙本质粘接强度低于健康牙本质，2.5% TiF 4溶液可显著提高酸蚀症牙本质的粘接强度。

牙齿酸蚀症;牙本质;牙齿粘接
ABSTRACT

ObjectiveTo investigate the effect of titanium tetrafluoride (TiF 4) solution on bonding strength of acid-etched dentin.

MethodsTooth crown dentin proximal to the pulp cavity was obtained from 24 freshly-extracted human third molars and randomly divided into 6 groups ( n=4 each) . Among them, 4 groups were modeled for artificial acid-etched dentin by means of acid-etching and mechanical friction. The other 2 groups were used as sound dentin controls. One each of the artificial acid-etched dentin groups and sound dentin control groups were randomly selected and studied under scanning electron microscope and microhardness tester to measure the surface morphology and hardness, and to evaluate the effectivity of the modeling for artificial acid-etched dentin. The other 3 groups of artificial acid-etched dentin and remaining group of healthy dentin were used for bonding test, re-assigned into 4 groups according to different treatments: etched dentin treated with 4.0% TiF 4 (4.0% TiF 4-ED group) , etched dentin treated with 2.5% TiF 4 (2.5% TiF 4-ED group) , untreated etched dentin (ED group) and sound dentin control (SD group) . All dentin samples were then bonded with a self-etching system (SE bond) , layered by AP-X composite resin with light-curing. Microtensile bonding strength (μTBS) test was used to measure the immediate bonding strength and to determine the fracture pattern.

ResultsThe microhardness values of artificial acid-eched dentin and sound dentin were (56.92±5.27) HV and (60.19±4.52) HV, respectively, with significant difference between groups ( P<0.05) . Scanning electron microscopy confirmed that the artificial acid-etched dentin presented microscopic morphological characteristics consistent to natural acid etching. The μTBS of 4.0% TiF 4-ED group, 2.5% TiF 4-ED group, ED group and SD group were (28.94±8.04) MPa, (46.08±8.19) MPa, (20.39±7.46) MPa and (45.16±7.86) MPa, respectively. The μTBS did not differ significantly between the 2.5% TiF 4-ED group and the SD group ( P=0.529) , and both were higher than those in the 4.0% TiF 4-ED group and the ED group (all P<0.05) . The fracture patterns differed significantly among the 4 groups ( χ 2=54.91, P<0.001) . In the 2.5% TiF 4-ED group, cohesive dentin fracture was the main cause, suggesting that the bonding strength may be higher than the mean measurements from the micro-tensile test.

ConclusionThe bonding strength of acid-etched dentin is lower compared with sound dentin. 2.5% TiF 4 solution can significantly improve the bonding strength of acid-etched dentin.

Acid-etched tooth;Dentin;Dental bonding
Tan Jianguo, Email: mocdef.aabnis.pivemuwnat
Copyright by Chinese Medical Association
No content published by the journals of Chinese Medical Association may be reproduced or abridged without authorization. Please do not use or copy the layout and design of the journals without permission.
All articles published represent the opinions of the authors, and do not reflect the official policy of the Chinese Medical Association or the Editorial Board, unless this is clearly specified.
引用本文

孙傲,马欣蓉,刘晓强,等. 四氟化钛溶液对酸蚀症牙本质粘接强度的影响[J]. 中华生物医学工程杂志,2021,27(01)：1-7.

DOI:10.3760/cma.j.cn115668-20200201-00019

PERMISSIONS

Request permissions for this article from CCC.

评价本文
*以上评分为匿名评价

版权归中华医学会所有。

未经授权，不得转载、摘编本刊文章，不得使用本刊的版式设计。

除非特别声明，本刊刊出的所有文章不代表中华医学会和本刊编委会的观点。

牙酸蚀症是指牙齿受到非细菌性酸的侵蚀，酸性物质对牙齿硬组织中的羟基磷灰石酸蚀脱矿，导致牙齿表面硬组织的进行性丧失 [ 1 ]。而酸蚀导致的牙齿表面硬组织脱矿软化，是牙齿磨耗的重要始动及促进因素 [ 2 ]。牙酸蚀症可以导致牙齿重度磨耗的发生 [ 3 ]，给患者带来诸如牙齿敏感不适、疼痛等症状，并引起咀嚼效能下降。造成牙齿侵蚀的酸可分为内源性酸及外源性酸，随着软饮料特别是碳酸饮料消费量日趋升高，牙酸蚀症的发病率正在快速提高，全球范围内青少年及成人牙酸蚀症的发病率已超过30% [ 4 , 5 , 6 ]。
近年来随着微创理念流行且技术日益发展，树脂粘接已经成为牙酸蚀症修复治疗的核心要素 [ 7 , 8 ]。当酸蚀仅累及牙釉质时，自酸蚀树脂粘接系统的粘接强度下降 [ 9 ]，而全酸蚀树脂粘接系统尚能提供临床可接受的粘接强度 [ 10 ]；但当酸蚀症进一步侵犯牙本质层而形成酸蚀症牙本质时，目前广泛使用的各类粘接系统及其相应产品则表现为较健康牙本质降低的粘接强度 [ 11 , 12 ]。当前应用广泛的通用型自酸蚀树脂粘接系统对酸蚀症牙本质的即刻粘接强度仅约为健康牙本质的一半，而当牙本质酸蚀合并机械力时粘接强度相比单纯酸蚀则有进一步下降的趋势 [ 12 ]，因此对酸蚀症牙本质粘接应做进一步研究。
四氟化钛（tetroflouride，TiF 4）属于金属氟化物的一种，TiF 4水溶液可与牙本质表面暴露的胶原纤维结合并固定，提高牙本质表面的硬度及弹性模量 [ 13 ]；它还可以在牙本质表面形成一层致密的二氧化钛氧化层，具有抗微渗漏、抗酸等作用，可用于酸蚀症的预防 [ 14 ]。Torres等 [ 15 ]及Basting等 [ 16 ]研究发现TiF 4可被添加至两步法自酸蚀系统的预处理剂中，并可稳定地存在且不影响对健康牙本质的粘接效果；Tranquilin等 [ 17 ]研究发现，在应用全酸蚀系统行健康牙本质粘接时，磷酸酸蚀后再涂布TiF 4溶液，可提高牙本质粘接强度。上述研究提示，TiF 4有望提高酸蚀症牙本质的粘接强度。
研究发现，0.1%~4%的TiF 4均可增强牙齿抗酸及抗龋能力 [ 18 , 19 , 20 ]，且在这一范围内浓度越高抗酸效果越好。在牙本质粘接领域，2.5%和4.0%（w/v）是两个常见的浓度 [ 12 , 15 , 16 , 17 ]，故本研究尝试采用2.5%及4.0%浓度的TiF 4溶液处理酸蚀症牙本质，观察其对酸蚀症牙本质粘接的影响，报告如下。
参考文献
[1]
The glossary of prosthodontic terms：ninth edition[J]. J Prosthet Dent， 2017，117(5S):e1-1e105. DOI： 10.1016/j.prosdent.2016.12.001 .
返回引文位置Google Scholar
百度学术
万方数据
[2]
Shellis RP , Addy M . The interactions between attrition，abrasion and erosion in tooth wear[J]. Monogr Oral Sci， 2014，25:32-45. DOI： 10.1159/000359936 .
返回引文位置Google Scholar
百度学术
万方数据
[3]
Loomans B , Opdam N , Attin T ，et al. Severe tooth wear：european consensus statement on management guidelines[J]. J Adhes Dent， 2017，19(2):111-119. DOI： 10.3290/j.jad.a38102 .
返回引文位置Google Scholar
百度学术
万方数据
[4]
Lussi A , Jaeggi T . Erosion—diagnosis and risk factors[J]. Clin Oral Investig， 2008，12(Suppl 1):S5-13. DOI： 10.1007/s00784-007-0179-z .
返回引文位置Google Scholar
百度学术
万方数据
[5]
Salas MM , Nascimento GG , Huysmans MC ，et al. Estimated prevalence of erosive tooth wear in permanent teeth of children and adolescents：an epidemiological systematic review and meta-regression analysis[J]. J Dent， 2015，43(1):42-50. DOI： 10.1016/j.jdent.2014.10.012 .
返回引文位置Google Scholar
百度学术
万方数据
[6]
Bartlett DW , Lussi A , West NX ，et al. Prevalence of tooth wear on buccal and lingual surfaces and possible risk factors in young European adults[J]. J Dent， 2013，41(11):1007-1013. DOI： 10.1016/j.jdent.2013.08.018 .
返回引文位置Google Scholar
百度学术
万方数据
[7]
王嘉德. 牙齿硬组织慢性损伤性疾病的病因研究与防治原则[J]. 中华口腔医学杂志， 2008，43(10):616-619. DOI： 10.3321/j.issn:1002-0098.2008.10.011 .
返回引文位置Google Scholar
百度学术
万方数据
[8]
骆小平. 牙齿磨损的病因、分类及修复重建治疗进展[J]. 中华口腔医学杂志， 2016，51(10):577-582. DOI： 10.3760/cma.j.issn.1002-0098.2016.10.001 .
返回引文位置Google Scholar
百度学术
万方数据
[9]
Casas-Apayco LC , Dreibi VM , Hipólito AC ，et al. Erosive cola-based drinks affect the bonding to enamel surface：an in vitro study[J]. J Appl Oral Sci， 2014，22(5):434-441. DOI： 10.1590/1678-775720130468 .
返回引文位置Google Scholar
百度学术
万方数据
[10]
Lenzi T , Hesse D , Guglielmi C ，et al. Shear bond strength of two adhesive materials to eroded enamel[J]. J Contemp Dent Pract， 2013，14(4):700-703. DOI： 10.5005/jp-journals-10024-1387 .
返回引文位置Google Scholar
百度学术
万方数据
[11]
Cruz JB , Bonini G , Lenzi TL ，et al. Bonding stability of adhesive systems to eroded dentin[J]. Braz Oral Res， 2015，29: 10.1590/1807-3107BOR-2015.vol29.0088 . DOI：.
返回引文位置Google Scholar
百度学术
万方数据
[12]
Augusto MG , Torres C , Pucci CR ，et al. Bond stability of a universal adhesive system to eroded/abraded dentin after deproteinization[J]. Oper Dent， 2018，43(3):291-300. DOI： 10.2341/16-173-L .
返回引文位置Google Scholar
百度学术
万方数据
[13]
Basting RT , Leme AA , Bridi EC ，et al. Nanomechanical properties，SEM，and EDS microanalysis of dentin treated with 2.5% titanium tetrafluoride，before and after an erosive challenge[J]. J Biomed Mater Res B Appl Biomater， 2015，103(4):783-789. DOI： 10.1002/jbm.b.33254 .
返回引文位置Google Scholar
百度学术
万方数据
[14]
Mantilla TF , Turssi CP , Ramos-Oliveira TM ，et al. The in situ effect of titanium tetrafluoride gel on erosion/abrasion progression in human dentin[J]. Braz Dent J， 2017，28(3):337-345. DOI： 10.1590/0103-6440201601247 .
返回引文位置Google Scholar
百度学术
万方数据
[15]
Torres GB , da Silva TM , Basting RT ，et al. Resin-dentin bond stability and physical characterization of a two-step self-etching adhesive system associated with TiF 4 [J]. Dent Mater， 2017，33(10):1157-1170. DOI： 10.1016/j.dental.2017.07.016 .
返回引文位置Google Scholar
百度学术
万方数据
[16]
Basting RT , Basting RT , Velarde Barrientos S ，et al. Titanium tetrafluoride incorporated into a two-step self-etching adhesive system：physico-mechanical characterization and bonding stability[J]. J Mech Behav Biomed Mater， 2017，75:197-205. DOI： 10.1016/j.jmbbm.2017.07.021 .
返回引文位置Google Scholar
百度学术
万方数据
[17]
Tranquilin JB , Bridi EC , Amaral FL ，et al. TiF 4 improves microtensile bond strength to dentin when using an adhesive system regardless of primer/bond application timing and method [J]. Clin Oral Investig， 2016，20(1):101-108. DOI： 10.1007/s00784-015-1496-2 .
返回引文位置Google Scholar
百度学术
万方数据
[18]
Castilho AR , Salomão PM , Buzalaf MA ，et al. Protective effect of experimental mouthrinses containing NaF and TiF 4 on dentin erosive loss in vitro [J]. J Appl Oral Sci， 2015，23(5):486-490. DOI： 10.1590/1678-775720150127 .
返回引文位置Google Scholar
百度学术
万方数据
[19]
Souza BM , Lima LL , Comar LP ，et al. Effect of experimental mouthrinses containing the combination of NaF and TiF 4 on enamel erosive wear in vitro [J]. Arch Oral Biol， 2014，59(6):621-624. DOI： 10.1016/j.archoralbio.2014.03.008 .
返回引文位置Google Scholar
百度学术
万方数据
[20]
Magalhães AC , Wiegand A , Rios D ，et al. Fluoride in dental erosion[J]. Monogr Oral Sci， 2011，22:158-170. DOI： 10.1159/000325167 .
返回引文位置Google Scholar
百度学术
万方数据
[21]
Magalhães AC , Levy FM , Souza BM ，et al. Inhibition of tooth erosion by milk containing different fluoride concentrations：an in vitro study[J]. J Dent， 2014，42(4):498-502. DOI： 10.1016/j.jdent.2013.12.009 .
返回引文位置Google Scholar
百度学术
万方数据
[22]
Wang YL , Chang HH , Chiang YC ，et al. Effects of fluoride and epigallocatechin gallate on soft-drink-induced dental erosion of enamel and root dentin[J]. J Formos Med Assoc， 2018，117(4):276-282. DOI： 10.1016/j.jfma.2018.01.020 .
返回引文位置Google Scholar
百度学术
万方数据
[23]
Schlueter N , Hara A , Shellis RP ，et al. Methods for the measurement and characterization of erosion in enamel and dentine[J]. Caries Res， 2011，45Suppl 1:13-23. DOI： 10.1159/000326819 .
返回引文位置Google Scholar
百度学术
万方数据
[24]
Ganss C , Hardt M , Blazek D ，et al. Effects of toothbrushing force on the mineral content and demineralized organic matrix of eroded dentine[J]. Eur J Oral Sci， 2009，117(3):255-260. DOI： 10.1111/j.1600-0722.2009.00617.x .
返回引文位置Google Scholar
百度学术
万方数据
[25]
Lussi A , Schlueter N , Rakhmatullina E ，et al. Dental erosion—an overview with emphasis on chemical and histopathological aspects[J]. Caries Res， 2011，45Suppl 1:2-12. DOI： 10.1159/000325915 .
返回引文位置Google Scholar
百度学术
万方数据
[26]
Ganss C , Lussi A , Schlueter N . The histological features and physical properties of eroded dental hard tissues[J]. Monogr Oral Sci， 2014，25:99-107. DOI： 10.1159/000359939 .
返回引文位置Google Scholar
百度学术
万方数据
[27]
Flury S , Koch T , Peutzfeldt A ，et al. The effect of a tin-containing fluoride mouth rinse on the bond between resin composite and erosively demineralised dentin[J]. Clin Oral Investig， 2013，17(1):217-225. DOI： 10.1007/s00784-012-0697-1 .
返回引文位置Google Scholar
百度学术
万方数据
[28]
Domingues LG , Real CM , Bridi EC ，et al. Effects of 2.5% TiF 4 on microtensile bond strength：Influence of application method and degree of dentin mineralization [J]. Int J Adhes Adhes， 2014，54(1):159-164. DOI： 10.1016/j.ijadhadh.2014.06.005 .
返回引文位置Google Scholar
百度学术
万方数据
备注信息
A
谭建国，Email： mocdef.aabnis.pivemuwnat
B
所有作者均声明不存在利益冲突
C
国家自然科学基金 (81701003)
评论 （0条）
注册
登录
时间排序
暂无评论，发表第一条评论抢沙发
最新推荐
更多
不同类型粘接剂对牙本质粘接强度及耐久性影响的比较研究
张文浩 等
国际医药卫生导报 2022,28(06)
氟化钠对人成骨肉瘤Saos-2细胞PI3K/Akt信号表达及凋亡的影响
蒋香菊 等
中华地方病学杂志 2021,40(03)
术前口服高糖溶液对胃癌手术患者术后恢复、HOMA-IR及血清hsCRP水平的影响
汪杰 等
中华内分泌外科杂志 2021,15(06)
氟化钠对仔鼠生长发育和血清氧化应激水平的影响
陈龙 等
中华地方病学杂志 2021,40(08)
MedAI助手（体验版）
文档即答
智问智答
机器翻译
回答内容由人工智能生成，我社无法保证其准确性和完整性，该生成内容不代表我们的态度或观点，仅供参考。
生成快照
文献快照

你好，我可以帮助您更好的了解本文，请向我提问您关注的问题。

0/2000

《中华医学会杂志社用户协议》 | 《隐私政策》

《SparkDesk 用户协议》 | 《SparkDesk 隐私政策》

网信算备340104764864601230055号 | 网信算备340104726288401230013号

技术支持：

历史对话
本文全部
还没有聊天记录
设置
模式
纯净模式沉浸模式
字号
小中大