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ENGLISH ABSTRACT
宏基因组高通量测序技术在革兰阴性菌耐药表型检测中的研究进展
梁文炎
施毅
孙文逵
作者及单位信息
·
DOI： 10.3760/cma.j.cn112147-20210705-00468
Progress of metagenomics next-generation sequencing in the detection of drug-resistant phenotypes of Gram-negative bacteria
Liang Wenyan
Shi Yi
Sun Wenkui
Authors Info & Affiliations
Liang Wenyan
Department of Respiratory & Critical Care Medicine, the First Affiliated Hospital of Nanjing Medical University, Nanjing 210029, China
Shi Yi
Department of Respiratory Medicine, Jinling Hospital, Nanjing University School of Medicine, Nanjing 210002, China
Sun Wenkui
Department of Respiratory & Critical Care Medicine, the First Affiliated Hospital of Nanjing Medical University, Nanjing 210029, China
·
DOI： 10.3760/cma.j.cn112147-20210705-00468
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摘要

耐药细菌感染的不断蔓延对公共卫生安全构成重要威胁，早期诊断和目标治疗是治疗成功的关键。传统细菌培养及药物敏感性检测耗时长、敏感性不高，无法满足临床诊治需求，高通量测序等分子检测技术成为新的发展方向。本文对近年来有关高通量测序在革兰阴性菌耐药诊治中的进展做一综述。

ABSTRACT

The ongoing spread of drug-resistant bacterial infections poses a significant threat to public health, and early diagnosis and targeted treatment are the keys to successful treatment. Traditional bacterial culture and drug susceptibility testing is time-consuming and insensitive, and cannot meet the needs of clinical diagnosis and treatment, so molecular detection technologies, such as metagenomics next-generation sequencing, have become a new research direction. This article reviewed recent advances in high-throughput sequencing for the diagnosis and treatment of drug resistance in Gram-negative bacteria.

Sun Wenkui, Email: nc.defudabe.umjniuknewnus
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引用本文

梁文炎,施毅,孙文逵. 宏基因组高通量测序技术在革兰阴性菌耐药表型检测中的研究进展[J]. 中华结核和呼吸杂志,2022,45(02)：209-213.

DOI:10.3760/cma.j.cn112147-20210705-00468

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细菌耐药现象日益常见且已成为全球公共卫生安全最大威胁之一 ［ 1 ］ 。临床实践过程中，广泛耐药革兰阴性菌（extensively drug resistant Gram-negative bacilli，XDR-GNB）作为院内常见复杂耐药菌受到关注，肺炎克雷伯菌、鲍曼不动杆菌、铜绿假单胞菌耐药现象尤为严重，已出现对所有抗菌药物耐药的超级菌种 ［ 2 , 3 , 4 , 5 , 6 ］ 。感染性疾病的精准治疗成为临床迫切要求。目前传统的细菌检测手段普遍存在耗时长、敏感性不高等不足，经验性使用广谱抗菌药物是初始抗感染治疗的常用方案。经验性抗感染治疗常导致药物的不恰当使用，不仅延误治疗，也会加重耐药现象。传统细菌及其耐药检测手段已无法满足现今医疗需求，寻找新的快捷、简便检测手段成为了迫切需求，分子检测手段就此进入舞台 ［ 7 , 8 ］ 。
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备注信息
A
孙文逵，Email： nc.defudabe.umjniuknewnus
B

梁文炎, 施毅, 孙文逵. 宏基因组高通量测序技术在革兰阴性菌耐药表型检测中的研究进展[J]. 中华结核和呼吸杂志, 2022, 45(2): 209-213. DOI: 10.3760/cma.j.cn112147-20210705-00468.

C
所有作者声明无利益冲突
D
国家自然科学基金 (81770009)
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