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ENGLISH ABSTRACT
基于体外分区囊袋模型的不同类型人工晶状体对晶状体上皮细胞迁移的抑制作用
廉飞玥
李阳
姜凌峰
沈皓月
赵江月
严肖啸
于佳明
秦宇
作者及单位信息
·
DOI： 10.3760/cma.j.cn115989-20200514-00346
Inhibition of lens epithelial cell migration using different square-edge intraocular lenses in an in vitro capsular bag model
Lian Feiyue
Li Yang
Jiang Lingfeng
Shen Haoyue
Zhao Jiangyue
Yan Xiaoxiao
Yu Jiaming
Qin Yu
Authors Info & Affiliations
Lian Feiyue
Department of Ophthalmology, the Fourth Affiliated Hospital of China Medical University, Key Lens Research Laboratory of Liaoning Province, Shenyang 110005, China
Li Yang
Department of Ophthalmology, the Fourth Affiliated Hospital of China Medical University, Key Lens Research Laboratory of Liaoning Province, Shenyang 110005, China
Jiang Lingfeng
Department of Ophthalmology, the Fourth Affiliated Hospital of China Medical University, Key Lens Research Laboratory of Liaoning Province, Shenyang 110005, China
Shen Haoyue
Department of Ophthalmology, the Fourth Affiliated Hospital of China Medical University, Key Lens Research Laboratory of Liaoning Province, Shenyang 110005, China
Zhao Jiangyue
Department of Ophthalmology, the Fourth Affiliated Hospital of China Medical University, Key Lens Research Laboratory of Liaoning Province, Shenyang 110005, China
Yan Xiaoxiao
Department of Ophthalmology, the Fourth Affiliated Hospital of China Medical University, Key Lens Research Laboratory of Liaoning Province, Shenyang 110005, China
Yu Jiaming
Department of Ophthalmology, the Fourth Affiliated Hospital of China Medical University, Key Lens Research Laboratory of Liaoning Province, Shenyang 110005, China
Qin Yu
Department of Ophthalmology, the Fourth Affiliated Hospital of China Medical University, Key Lens Research Laboratory of Liaoning Province, Shenyang 110005, China
·
DOI： 10.3760/cma.j.cn115989-20200514-00346
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摘要

目的构建体外分区囊袋模型，探讨不同类型360°连续直角边缘人工晶状体(IOL)对晶状体上皮细胞(LECs)迁移的抑制作用。

方法使用Transwell小室、细胞爬片、人源Ⅳ型胶原、IOL建立后发性白内障(PCO)体外分区囊袋模型作为模型组，并设置一个空白处理的Transwell小室作为对照组。人LECs细胞系SRA01/04培养于各组Transwell小室中，应用倒置显微镜观察各组中PCO早期病理表现；采用苏木精-伊红染色观察各组细胞形态改变。根据体外分区囊袋模型植入的IOL类型分为平台板式袢HydroSmart组、C型袢HydroSmart组、C型补偿袢Hydrophobic组，并设置不植入IOL为空白对照组。应用Transwell法检测各组IOL前囊膜区迁移细胞数目并计算前囊膜细胞迁移抑制率；应用细胞排斥区分析法检测各组IOL后囊膜区细胞迁移数目并计算后囊膜细胞迁移抑制率。

结果模型组细胞培养48 h后体外分区囊袋模型后囊膜区可见细胞形成类似早期Soemmering环和小型Elschnig珍珠样小体等PCO特征性病理表现；苏木精-伊红染色显示模型组迁移的细胞呈纤维状。对照组中均未见类似细胞分布及形态改变。平台板式袢HydroSmart组、C型袢HydroSmart组、C型补偿袢Hydrophobic组前囊膜区域单位面积迁移细胞计数分别为18.80±5.53、24.67±9.80和34.47±10.80，后囊膜袢光学部移行区迁移细胞计数分别为56.43±9.00、162.20±16.38和121.30±12.01，IOL前囊膜细胞迁移抑制率分别为(92.02±1.94)%、(89.76±3.10)%和(86.27±4.54)%；后囊膜细胞迁移抑制率分别为(91.60±3.65)%、(70.14±5.35)%和(78.43±3.48)%。平台板式袢HydroSmart组前囊膜区迁移细胞数目明显少于C型补偿袢Hydrophobic组，细胞迁移抑制率明显高于C型补偿袢Hydrophobic组，差异均有统计学意义(均 P<0.05)；平台板式袢HydroSmart组后囊膜袢光学部移行区迁移细胞数、细胞迁移抑制率明显高于C型袢HydroSmart组和C型补偿袢Hydrophobic组，差异均有统计学意义(均 P<0.001)。

结论成功构建体外分区囊袋模型。与C型袢HydroSmart IOL、C型补偿袢Hydrophobic IOL相比，HydroSmart平台板式袢IOL能更有效抑制LECs的迁移。

白内障;后囊膜混浊;细胞迁移抑制;人工晶状体;晶状体上皮细胞;体外囊袋模型
ABSTRACT

ObjectiveTo establish an in vitro capsular bag model and compare the inhibitory effects of different 360° square-edge intraocular lens (IOL) on lens epithelial cells (LECs) migration.

Methods In vitro capsular bag model with posterior capsule opacification (PCO) was established using Transwell compartment, cell climbing slices, human collagen type Ⅳ, and IOL.The models were divided into Plate-loop HydroSmart group, C-loop HydroSmart group, and C-compensation-loop Hydrophobic group according to the different square-edge IOL implanted.A blank control group was set using the Transwell compartment without IOL.The early PCO pathological manifestations in lens epithelial cell line SRA01/04 cultured in the Transwell compartment were observed with an inverted microscope.The cell morphology in different groups was observed by hematoxylin and eosin staining.The cell counting and cell migration inhibition rate of anterior capsule and posterior capsule were calculated by Transwell assay and cell-exclusion zone assay, respectively.

ResultsThe early pathological characteristics of PCO, such as early Soemmering ring and small Elschnig pearl, could be found in cells in the in vitro capsular bag model after 48-hour culture.The migrating cells in model groups were fibrous.No changes mentioned above were found in blank control group.The number of migrating cells in the anterior capsule of Plate-loop HydroSmart group, C-loop HydroSmart group, C-compensation-loop Hydrophobic group was 18.80±5.53, 24.67±9.80, and 34.47±10.80, respectively, and the number of migrating cells in the optical area of the posterior capsule of the three groups was 56.43±9.00, 162.20±16.38, and 121.30±12.01, respectively.The cell migration inhibition rate in the anterior capsule of Plate-loop HydroSmart group, C-loop HydroSmart group, C-compensation-loop Hydrophobic group was (92.02±1.94)%, (89.76±3.10)%, (86.27±4.54)%, respectively, and the cell migration inhibition rate in optical area of the posterior capsule of the three groups was (91.60±3.65)%, (70.14±5.35)%, (78.43±3.48)%, respectively.The number of migrating cells in the anterior capsule was lower and the cell migration rate inhibition was higher in Plate-loop HydroSmart group than C-compensation-loop Hydrophobic group, with significant differences (both at P<0.05). The number of migrating cells in the optical area of the posterior capsule and the cell migration inhibition rate was greater than those of C-loop HydroSmart group and C-compensation-loop Hydrophobic group, showing statistically significant differences (all at P<0.001).

ConclusionsThe in vitro capsular bag model can be used in PCO research.Compared with C-loop HydroSmart IOL and C-compensation-loop Hydrophobic IOL, Plate-loop HydroSmart IOL can more effectively inhibit the migration of LECs to the optical area of the posterior capsule.

Cataract;Capsule opacification;Cell migration inhibition;Lenses, intraocular;Lens epithelial cells; In vitro capsular bag model
Qin Yu, Email: mocdef.6ab21bbuyniq
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引用本文

廉飞玥,李阳,姜凌峰,等. 基于体外分区囊袋模型的不同类型人工晶状体对晶状体上皮细胞迁移的抑制作用[J]. 中华实验眼科杂志,2022,40(05)：395-402.

DOI:10.3760/cma.j.cn115989-20200514-00346

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后发性白内障(posterior capsular opacification，PCO)是白内障术后严重影响视觉质量的并发症之一，目前主要治疗方式为YAG激光和二次手术。随着患者对术后视觉质量要求的不断提高，激光或手术治疗带来的人工晶状体(intraocular lens，IOL)损伤或移位等并发症逐渐得到重视。已有研究表明，360°连续直角边缘IOL能有效抑制晶状体上皮细胞(lens epithelial cells，LECs)向后囊膜迁移，而不同360°连续直角边缘IOL的PCO发生率存在差异 [ 1 ]。目前，关于不同360°连续直角边缘IOL在PCO预防中作用的体外实验研究仍然较少，其中一部分原因可能在于囊袋模型构建的限制 [ 2 , 3 , 4 ]。早期囊袋模型是由动物眼球囊袋及人晶状体囊袋构建，除囊袋来源受限外，模型的稳定性也较差 [ 5 , 6 , 7 , 8 , 9 , 10 , 11 ]。近年来，使用细胞培养小室构建PCO体外囊袋模型的新方法出现 [ 12 , 13 , 14 ]，但该类模型尚无法准确模拟术后晶状体囊内IOL与前、后囊膜的位置关系，缺乏对囊袋不同部位LECs状态的反映，这些问题也是PCO预防研究的关键 [ 15 , 16 , 17 , 18 ]。本研究拟设计体外分区囊袋模型，为现有体外囊袋模型存在的问题提出可能的解决方案。同时，本研究拟使用体外分区囊袋模型比较目前常用的3种类型360°连续直角边缘IOL对LECs迁移的抑制作用，为IOL改良以预防PCO提供新的参考和依据。
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备注信息
A
秦宇，Email： mocdef.6ab21bbuyniq
B

廉飞玥：实施研究、论文撰写；李阳：实施研究；姜凌峰、沈皓月、严肖啸：数据整理、统计分析；赵江月、于佳明：论文修改；秦宇：酝酿和设计实验、研究指导、论文修改及定稿

C
本研究曾受到蔡司、高视远望和眼力健公司的资助，所有作者均声明不存在其他利益冲突
D
国家自然科学基金青年基金项目 (81600717)
辽宁省自然科学基金项目 (201602851、2021-MS-200)
"双一流"国家自然科学基金培育项目 (114-3111210504)
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