目的建立小鼠星状神经节阻滞(SGB)模型，并探讨SGB对脑皮质血流量的影响。
方法清洁级健康雄性C57BL/6小鼠30只，8~9周龄，体重23~27 g，采用随机数字表法分为5组( n＝6)：对照组(C组)、左侧SGB组(L组)、左侧生理盐水组(SL组)、右侧SGB组(R组)及右侧生理盐水组(SR组)。C组不予任何处理；L组和R组采用0.25%罗哌卡因0.08 ml行经皮后入路SGB；SL组和SR组注射等量生理盐水。于SGB前(T 0)及SGB后10、30、60、90、120 min(T 1~5)时，采用激光散斑血流成像系统检测脑皮质血流量。
结果L组和R组小鼠均出现阻滞侧上睑下垂，提示小鼠SGB模型制备成功。C组、SL组和SR组各时点脑皮质血流量差异无统计学意义( P>0.05)，L组和R组阻滞侧脑皮质血流量T 1时降低，T 2时开始升高，T 3时达峰值，T 5时降低，但仍高于T 0时( P<0.01)；与C组和SL组比较，L组左侧脑皮质血流量T 1时降低，T 2~5时升高( P<0.01)；与C组和SR组比较，R组右侧脑皮质血流量T 1时降低，T 2~5时升高( P<0.01)；C组与SL组间、C组与RL组间各时点脑皮质血流量差异无统计学意义( P>0.05)。
结论本研究成功建立了小鼠经皮后入路SGB模型。单侧SGB可对阻滞侧脑皮质血流量产生影响，表现为短暂降低之后持续性升高。
ObjectiveTo develop a model of stellate ganglion block (SGB) in mice and investigate the effect of SGB on cerebral cortical blood flow.
MethodsThirty clean-grade healthy male C57BL/6 mice, aged 8-9 weeks, weighing 23-27 g, were divided into 5 groups ( n=6 each) using a random number table method: control group (group C), left SGB group (group L), left normal saline group (group SL), right SGB group (group R) and right normal saline group (group SR). Group C received no intervention.SGB was performed with 0.25% ropivacaine 0.08 ml via percutaneous posterior approach in L and R groups, while the equal volume of normal saline 0.08 ml was given instead at the location of left and right stellate ganglion in SL and SR groups, respectively.The cerebral cortical blood flow was determined using laser speckle contrast imaging system before SGB (T 0) and at 10, 30, 60, 90 and 120 min after SGB (T 1-5).
ResultsMice developed ptosis on the block side, indicating that the model of SGB was successfully developed in L and R groups.There was no significant difference in cerebral cortical blood flow at each time point among C, SL and SR groups ( P>0.05), and cerebral cortical blood flow on the block side decreased at T 1, began to increase at T 2, peaked at T 3, and decreased at T 5 which was still higher than that at T 0 in group L and group R ( P<0.01). Compared with C and SL groups, the left cerebral cortical blood flow was significantly decreased at T 1, 5 and increased at T 2-4 in group L ( P<0.01). Compared with C and SR groups, the right cerebral cortical blood flow was significantly decreased at T 1, 5 and increased at T 2-4 in group R ( P<0.01). There were no significant differences in cerebral cortical blood flow at each time point between group C and group SL and between group C and group RL ( P>0.05).
ConclusionsThe mouse model of SGB via percutaneous posterior approach is successfully developed.Unilateral SGB can affect cerebral cortical blood flow on the block side, which shows a transitory decrease followed by a sustained significant increase.
王嘉华,周炜,王小洪,等. 小鼠星状神经节阻滞模型的建立及其对脑皮质血流量的影响[J]. 中华麻醉学杂志,2022,42(04)：430-434.
DOI:10.3760/cma.j.cn131073.20211129.00411版权归中华医学会所有。
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王嘉华：实验设计和操作、数据采集和整理、统计学分析、论文撰写；周炜：实验设计、统计学分析、论文修改；王小洪：实验设计和指导；严诗婷和田顺平：数据采集；王莹：数据采集和整理；于乐洋和李虎：统计学分析；张东生：实验设计和指导；张转：实验设计和指导、统计学分析、论文撰写和修改、经费支持；刘微丽：脑血流数据分析和指导
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