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富血小板纤维蛋白制备和保存技术研究进展
施景源
李同汉
赵鸿
作者及单位信息
·
DOI： 10.3760/cma.j.cn115396-20230213-00026
Research progress on platelet-rich fibrin preparation and preservation technology
Shi Jingyuan
Li Tonghan
Zhao Hong
Authors Info & Affiliations
Shi Jingyuan
Department of Traumatic Surgery, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China
Li Tonghan
Department of Traumatic Surgery, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China
Zhao Hong
Department of Traumatic Surgery, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China
·
DOI： 10.3760/cma.j.cn115396-20230213-00026
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摘要

富血小板纤维蛋白(PRF)是一种具有立体纤维蛋白结构、富含大量白细胞和血小板的第二代血小板浓缩物，可持续释放各种促进伤口愈合的细胞因子及生长因子。PRF已在创伤外科、整形外科及口腔科等临床科室广泛用于治疗患者各种急慢性创面。对创伤外科中常见的慢性难愈合创面，常规的外科处理常效果不佳。大量的研究已证实PRF对慢性创面具有良好的治疗效果。根据标本选择、离心方法、离心管材质、冻干保存技术等多种方法，研究者尝试对PRF进行改良增效，并已取得巨大进展。本文就PRF制备和保存技术研究现状进展作一综述。

生物相容性材料;富血小板纤维蛋白;离心法;冷冻干燥法;伤口愈合
ABSTRACT

Platelet-rich fibrin(PRF), a second-generation of platelet concentrate with stereo fibrin structure, leukocyte and platelets, can release various cytokines and growth factors to promote wound healing. PRF has been widely used in the treatment of various acute and chronic wounds in traumatic surgery, plastic surgery and stomatology. Chronic wounds are common in traumatic surgery, but conventional treatment is often ineffective. Accumulating evidences have confirmed that PRF has excellent therapeutic effect on chronic wounds. According to various methods such as different specimen, centrifugation, centrifuge tube material, and freeze-drying, the researchers tried to improve the efficiency of PRF. The progress of platelet-rich fibrin preparation technology and preservation technology are reviewed in this paper.

Biocompatible materials;Platelet-rich fibrin;Centrifugation;Freeze drying;Wound healing
Zhao Hong, Email: mocdef.labiamtohoahz.gnoh.rd
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引用本文

施景源,李同汉,赵鸿. 富血小板纤维蛋白制备和保存技术研究进展[J]. 国际外科学杂志,2023,50(03)：212-216.

DOI:10.3760/cma.j.cn115396-20230213-00026

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富血小板纤维蛋白(Platelet-rich fibrin，PRF)是一种具有立体纤维蛋白结构、富含大量白细胞和血小板的第二代血小板浓缩物，可持续释放各种促进伤口愈合的细胞因子及生长因子。PRF制备方案由发明者Choukroun等[ 1 ]于2001年提出。采集10 mL静脉血于不含抗凝剂的试管中，并立即以3 000 r/min(相对离心力约为400×g)离心10 min。离心后血液在离心管中分为3层，中间层即为PRF凝胶。与第一代血小板浓缩物相比，具有制备过程更快速简单、无需添加任何凝血酶、抗凝剂等化学制剂，无免疫排斥反应和传播感染性疾病风险等诸多优点。研究显示，PRF可以通过促进血管生成、控制创面感染、分泌促创面愈合生长因子、加快瘢痕组织形成等方式参与创面修复[ 2 , 3 ]。目前PRF已广泛在口腔科、整形外科、创伤外科中应用，具有良好的治疗效果。改进PRF的相关研究一直是创面修复领域的热点，本文将就PRF制备技术及保存技术研究现状进行综述。
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赵鸿，Email: mocdef.labiamtohoahz.gnoh.rd
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