目的研究干眼患者与非干眼患者眼表微生物菌群的差异。
方法纳入2020年6—11月于西安交通大学第一附属医院确诊的干眼患者42例42眼作为干眼组，纳入同期在眼科门诊查体的非干眼患者37例37眼作为非干眼组，采集结膜囊拭子并测序。纳入符合标准单眼，若双眼符合纳入标准，则右眼入组。采用Miseq PE301+8+301平台进行细菌16S rRNA基因V3-V4区双端测序，对微生物菌群进行可操作分类种属(OTUs)聚类，分析和比较2个组间微生物菌群Alpha和Beta多样性，并进行物种注释分析以及微生物标志物分析。
结果共获得18 586个OTUs，干眼组与非干眼组共有OTUs为3 674个。Alpha多样性分析结果显示，2个组间observed species指数、Chao指数、Ace指数、Shannon指数和Simpson指数比较差异均无统计学意义(均P>0.05)，表明2个组间菌群丰富度无明显差异。而主成因(PCoA)分析显示2个组间菌群构成差异有统计学意义(R2＝0.039，F＝3.100，P＝0.022)。2个组眼表优势菌群构成相似，在门水平以变形菌、放线菌、厚壁菌、拟杆菌、蓝藻菌等为主，在属水平以嗜糖假单胞菌、棒状杆菌、丙酸杆菌、假单胞菌、草螺菌等为主。LEfSe分析显示干眼组优势菌属的标志菌为泰氏菌属、栖水菌属、芬戈尔德菌，非干眼组为柄杆菌属、弯钩菌属。
结论干眼组和非干眼组眼表微生物菌群构成存在差异。
ObjectiveTo explore the difference in ocular surface microbiota between patients with and without dry eye.
MethodsForty-two patients (42 eyes) diagnosed with dry eye were enrolled as dry eye group in the First Affiliated Hospital of Xi'an Jiaotong University from June to November 2020, and 37 controls without dry eye (37 eyes) were enrolled as control group in the same period.One eye was selected as the study eye, and the right eye was included when both eyes met the inclusion criteria.Swab samples from the conjunctival sac were obtained and sequenced.Sequencing of the V3-V4 region of the bacterial 16S rRNA gene was performed with Miseq PE301+ 8+ 301 platform.Operational taxonomic species (OTUs) clustering of microflora, comparison of alpha and beta diversity analysis of microflora between the two groups, annotation analysis of species and analysis of microbial markers were performed.The study protocol was approved by the Ethics Committee of The First Affiliated Hospital of Xi'an Jiaotong University (No.XJTU1AFCRC2018SJ-014). Written informed consent was obtained from each subject before any medical examination.
ResultsA total of 18 586 OTUs were obtained, and 3 674 OTUs were shared between the two groups.Alpha diversity analysis showed that there was no significant difference in observed species index, Chao index, Ace index, Shannon index and Simpson index between the two groups (all at P>0.05), suggesting there was no difference in microbiota richness between them.The PCoA analysis showed that the microbial compositions of the two groups were significantly different (R2=0.039, F=3.100, P=0.022). The dominant flora of the two groups was similar, with Proteobacteria, Actinobacteria, Firmicutes, Bacteroidetes and Cyanobacteria as the top 5 abundant bacterial phyla, with Pelomonas, Corynebacterium, Propionibacterium, Pseudomonas and Herbaspirillum as the top 5 bacterial genera.LEfSe analysis identified Tissierellaceae, Enhydrobater and Finegoldia as dominant bacterial genera in dry eye group, and Caulobacter and Curvibacter in control group.
ConclusionsThe composition of ocular surface microbiomes is different between dry eye patients and controls.
宋瑜,齐赟,张明,等. 干眼患者与非干眼患者眼表微生物菌群差异分析[J]. 中华实验眼科杂志,2023,41(05)：436-441.
DOI:10.3760/cma.j.cn115989-20211228-00718版权归中华医学会所有。
未经授权，不得转载、摘编本刊文章，不得使用本刊的版式设计。
除非特别声明，本刊刊出的所有文章不代表中华医学会和本刊编委会的观点。
宋瑜：参与研究实施、数据采集、分析、论文撰写；齐赟：参与患者招募、研究实施；张明：参与研究实施、数据采集及分析；康前雁：参与实验设计、患者诊断及招募、数据验证及论文修改；黎黎：参与实验设计、对文章智力性内容的审阅和修改及论文最终定稿
文档即答
智问智答
机器翻译
你好，我可以帮助您更好的了解本文，请向我提问您关注的问题。
