目的探索一种编程数字化技术和数学几何原理相结合对豚鼠眼球形态学参数进行量化分析的方法。
方法选取22只3周龄清洁级雄性三色豚鼠，采用过量麻醉法处死并取出眼球，采用1 300万像素微距仪的高拍模式进行水平面及矢状面拍照，图片导入pycharm编程软件。应用Python 3.9预先编写好的分析程序，先通过刻度尺获取图片像素与实际距离的换算系数，再对角膜表面进行圆弧拟合及圆锥曲线拟合。圆弧拟合后的结果经换算后计算出豚鼠的角膜曲率半径；通过圆锥曲线通用方程拟合(Ax 2+Bxy+Cy 2+Dx+Ey+F＝0)，得出角膜表面的离心率e值；通过对全角膜及中央区3 mm的拟合，评估角膜的非球面性质。
结果应用Python编程数字化方法可以完整清晰地展现豚鼠的角膜轮廓。横切面上，数字化拟合中央3 mm、数字化拟合全角膜以及曲率计测量全角膜曲率比较，差异无统计学意义( F＝1.693， P＝0.190)；矢状面上，3种方法测得的角膜曲率比较，差异有统计学意义( F＝3.500， P＝0.030)，其中曲率计测量全角膜的角膜曲率明显大于数字化拟合全角膜，差异有统计学意义( P<0.05)。横切面和矢状面上，3种方法测得的角膜曲率半径比较，差异均无统计学意义( F＝1.817， P＝0.170； F＝2.050， P＝0.133)。离心率测量结果显示，横切面和矢状面数字化拟合中央区3 mm处e值分别为0.55±0.15和0.53±0.17，分别低于数字化拟合全角膜e值的0.66±0.10和0.64±0.14，差异均有统计学意义( t＝－4.860、－5.210，均 P<0.01)。
结论应用Python编程数字化方法测量豚鼠角膜曲率及离心率切实可行。
ObjectiveTo investigate an accurate and quantitative method to measure the eyeball morphological parameters of guinea pigs through a method that combines programmed digital techniques and mathematical geometric principles.
MethodsTwenty-two three-week-old clean-grade male tricolor guinea pigs were selected and sacrificed by anesthesia overdose.Eyeballs were enucleated.The horizontal and sagittal images of the eyeball were taken with the high-speed photographic model of 13 million pixels macro meter, and the pictures were imported into pycharm programming software.Using the pre-written analysis program of Python 3.9, the conversion coefficient between the photo pixel and the actual length was obtained by a scale, and then the corneal surface was fitted by arc fitting and conic curve fitting.The results of arc fitting were converted to calculate the corneal radius of curvature.The corneal eccentricity was calculated according to the general conic equation (Ax 2+ Bxy+ Cy 2+ Dx+ Ey+ F=0). The corneal asphericity was evaluated by curve fitting between the central 3-mm and the whole cornea.The use and care of the animals complied with Regulations for the Administration of Affairs Concerning Experimental Animals by State Science and Technology Commission.The study protocol was approved by the Ethics Committee of Shanghai Public Health Clinical Center (No.2022-A009-01).
ResultsThe digital method of Python programming can show the corneal contour of guinea pigs completely and clearly.In the transverse plane, there was no significant difference in the corneal curvature measurements among the digital fitting in central 3-mm cornea, digital fitting in whole cornea and curvature meter ( F=1.693, P=0.190). In the sagittal plane, there was a significant difference in the corneal curvature measurements among the three methods ( F=3.500, P=0.030), and the corneal curvature measurements of the whole cornea measured by the curvature meter were significantly greater than those measured by the digital fitting ( P<0.05). There was no statistical significance in the measurements of corneal curvature radius among the three methods in the transverse plane and the sagittal plane ( F=1.817, P=0.170; F=2.050, P=0.133). The horizontal and sagittal corneal eccentricity measured by digital fitting in central 3-mm cornea were 0.55±0.15 and 0.53±0.17, which were lower than 0.66±0.10 and 0.64±0.14 measured by digital fitting in whole cornea, and the differences were statistically significant ( t=-4.860, -5.210; both at P<0.01).
ConclusionsIt is feasible to use Python programming digital method to measure the corneal curvature and eccentricity of guinea pigs.
邸悦,李昕悦,方王怡,等. 应用Python编程数字化测量豚鼠角膜曲率及离心率[J]. 中华实验眼科杂志,2023,41(08)：724-729.
DOI:10.3760/cma.j.cn115989-20220727-00349版权归中华医学会所有。
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除非特别声明，本刊刊出的所有文章不代表中华医学会和本刊编委会的观点。
邸悦：参与选题、设计实验；李昕悦、方王怡：分析数据、起草文章；黄颖、乔中豹：采集数据；骆文婷、叶海昀：实施研究；乔彤：审阅文章及定稿
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