目的探明活性炭在不同条件下对喹硫平吸附的影响，构建活性炭对喹硫平吸附效果的评价方法，评价活性炭在体外和体内对喹硫平吸附效果。
方法体外实验通过活性炭在不同有机溶剂含量、有机溶剂类型、吸附温度、吸附时间以及pH值等条件与喹硫平共孵育，平衡、离心后取上清，用LC-MS/MS检测上清中喹硫平的浓度，计算吸附率。以Log10活性炭浓度作为自变量，活性炭对喹硫平的吸附率为因变量，通过Origin 2021软件进行函数拟合。体内实验大鼠经灌胃给予喹硫平后，实验组灌胃给予125 mg/mL的活性炭，给药前、给药后0.17 h, 0.33 h, 0.50 h, 0.75 h, 1 h, 1.5 h, 2 h, 4 h, 6 h, 12 h, 24 h眼眶采血，血浆样本前处理后用LC-MS/MS检测喹硫平的浓度，通过DAS 2.0软件计算对照组及实验组中喹硫平的药代动力学参数。
结果有机溶剂含量、吸附温度、吸附时间以及pH值等因素影响活性炭对喹硫平的清除效果，最终优化选择统一的体外吸附实验方法。拟合了100个不同的吸附函数模型，筛选出Boltzmann函数是活性炭对喹硫平的最优吸附函数模型。药代动力学结果显示，与对照组相比，给予活性炭的实验组喹硫平C max和AUC显著降低。
结论：体外与体内实验结果表明，活性炭在体外与体内均对喹硫平具有一定的吸附效果。
ObjectiveTo investigate the impact of various conditions on the adsorption of quetiapine by activated carbon, establish a method for evaluating the adsorption efficacy of activated carbon on quetiapine, and assess the adsorption effects both in vitro and in vivo.
MethodsIn vitro experiments involved incubating activated carbon with quetiapine under different conditions, including varying organic solvent contents, types of organic solvents, adsorption temperatures, adsorption times, and pH. After reaching equilibrium, the mixtures were centrifuged, and the supernatants were collected. The concentration of quetiapine in the supernatants was measured using LC-MS/MS, and the adsorption rates were calculated. The log-transformed concentration of activated carbon was used as the independent variable and the adsorption rate as the dependent variable for function fitting using Origin 2021 software. In the in vivo experiments, rats were administered quetiapine orally, followed by 125 mg/mL of activated carbon in the experimental group. Blood samples were collected at multiple time points pre- and post-administration (0.17 h, 0.33 h, 0.50 h, 0.75 h, 1 h, 1.5 h, 2 h, 4 h, 6 h, 12 h, and 24 h). Plasma samples were pre-treated and the quetiapine concentrations were determined using LC-MS/MS. Pharmacokinetic parameters for both control and experimental groups were calculated using DAS 2.0 software.
ResultsThe factors such as organic solvent content, type of organic solvent, adsorption temperature, adsorption time, and pH value significantly influenced the adsorption efficiency of quetiapine by activated carbon, leading to the optimization and standardization of the in vitro adsorption methodology. Among the 100 different adsorption function models tested, the Boltzmann function was identified as the most suitable models for describing the adsorption of quetiapine by activated carbon. Pharmacokinetic analysis showed that the experimental group treated with activated carbon exhibited significantly reduced C max and AUC for quetiapine compared to the control group.
ConclusionThe results of both in vitro and in vivo experiments demonstrate that activated carbon effectively adsorbs quetiapine, providing a potential method for mitigating quetiapine absorption.
巴根,曹德才,石齐芳,等. 活性炭对喹硫平吸附效果的体外和大鼠体内评价方法研究[J]. 中华急诊医学杂志,2025,34(03)：335-341.
DOI:10.3760/cma.j.issn.1671-0282.2025.03.009版权归中华医学会所有。
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除非特别声明，本刊刊出的所有文章不代表中华医学会和本刊编委会的观点。
巴根：论文撰写、实验操作；曹德才、石齐芳、张孛妍：数据收集、整理和分析；谢未然：样本处理；毛征生：提供研究经费、研究设计、论文修改；张劲松、孙昊：文章指导
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