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结膜上皮干细胞研究进展
姚钦科
傅瑶 [综述]
作者及单位信息
·
DOI： 10.3760/cma.j.issn.2095-0160.2015.09.016
Research progress in conjunctival epithelial stem cells
Yao Qinke
Fu Yao
Authors Info & Affiliations
Yao Qinke
Department of Ophthalmology, Department of Ophthalmology, Shanghai Ninth People's Hospital, Shanghai Jiaotong University School of Medicine, Shanghai 200011, China
Fu Yao
·
DOI： 10.3760/cma.j.issn.2095-0160.2015.09.016
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摘要

结膜上皮干细胞是结膜上皮细胞和杯状细胞的共同祖细胞，具有较强的自我更新、增生和分化能力，在维持眼表稳态和损伤修复中起关键作用。随着对结膜上皮干细胞研究的不断深入，我们对眼表稳态的调节机制更加了解，并且为组织工程技术重建结膜上皮提供了种子细胞。本文就结膜上皮干细胞的概念、生物学特性、解剖学部位、鉴别、体外培养及其检测方法、分化、临床应用等的研究进展进行综述。

干细胞/细胞学;结膜上皮;杯状细胞;细胞培养
ABSTRACT

Conjunctival epithelial stem cells are the common progenitor cells of both conjunctival epithelial cells and goblet cells, having the ability of self-renewal proliferation and differentiation. They play a central role in maintaining the homeostasis of ocular surface and damage repair. With the in-depth study of conjunctival epithelial stem cells, we can further understand their effective mechanism in regulating ocular surface homeostasis. Furthermore, conjunctival epithelial stem cells are good candidates as seed cells for reconstructing conjunctiva in tissue engineering. The research progress in conjunctival epithelial stem cells, such as their concept, features, location, identification, culture method in vitro, differentiation and clinical application were reviewed.

Stem cell/cytology;Epithelial, conjunctiva;Goblet cells;Cell culture
FuYao, Email: mocdef.aabnisyfoayuf
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引用本文

姚钦科,傅瑶. 结膜上皮干细胞研究进展[J]. 中华实验眼科杂志,2015,33(9)：840-843.

DOI:10.3760/cma.j.issn.2095-0160.2015.09.016

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干细胞是具有自我更新能力的多潜能细胞，即保持未定向分化状态和具有增生的能力。根据干细胞所处的分化阶段可分为胚胎干细胞和成体干细胞；根据分化潜能的大小，干细胞又可分为全能干细胞、多能干细胞和单能干细胞。眼表存在2种干细胞，即角膜缘干细胞和结膜上皮干细胞，它们属于单能干细胞，这类干细胞仅向一种类型或者密切相关的2种类型细胞分化 [ 1 ]。角膜缘干细胞是角膜上皮细胞再生的源泉，对于维持角膜的透明和眼表的健康起着重要作用。结膜上皮由结膜上皮细胞和杯状细胞组成，其结构的完整和功能的正常对于维持眼表健康也起着非常重要的作用。目前，关于角膜缘干细胞的研究已经相对成熟并取得丰硕成果，但结膜上皮干细胞的研究尚待进一步深入。本文对结膜上皮干细胞的概念、解剖位置、体外培养及其检测方法、临床应用等进行综述。
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备注信息
A
傅瑶，Email： mocdef.aabnisyfoayuf
B
国家自然科学基金项目 (81370992)
上海市科委基础研究重点项目连续支持项目 (11JC1407000)
上海市浦江人才计划项目 (12PJD025)
上海交通大学医学院博士创新基金项目 (BXJ201227)
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