高级检索
中华实验眼科杂志
期刊首页
过刊列表
高级检索
稿件发表
• 实验研究 •
ENGLISH ABSTRACT
莱菔硫烷对体外培养牛眼小梁细胞硫氧还蛋白表达的影响及其机制
苏静
王强
刘玉震
作者及单位信息
·
DOI： 10.3760/cma.j.issn.2095-0160.2016.06.006
Effects of sulforaphane on thioredoxin expression in bovine trabecular meshwork cells
Su Jing
Wang Qiang
Liu Yuzhen
Authors Info & Affiliations
Su Jing
Binzhou Medical University Clinical Medicine College, Binzhou 256603, China
Wang Qiang
Department of Ophthalmology, Affiliated Hospital of Binzhou Medical University, Binzhou 256600, China
Liu Yuzhen
Binzhou Medical University Clinical Medicine College, Binzhou 256603, China
·
DOI： 10.3760/cma.j.issn.2095-0160.2016.06.006
343
10
0
0
0
0
PDF下载
APP内阅读
摘要

背景研究证实莱菔硫烷(SFN)可以激活多条通路，促进机体内抗氧化蛋白的表达。硫氧还蛋白(Trx)是维持细胞内氧化还原稳态的重要抗氧化蛋白之一。

目的研究SFN对体外培养的牛眼小梁细胞内Trx表达的影响及其机制。

方法体外培养牛眼小梁细胞并进行形态学观察，取第3代培养的牛眼小梁细胞，分别加入终浓度为0、10、20和30 μmol/L SFN培养30 min，应用实时荧光定量PCR技术检测细胞内Trx mRNA的表达。将细胞按照随机抽样的方法分为6个组，即空白对照组、LY294002组、U0126组、SFN组、LY294002＋SFN组和U0126＋SFN组。采用Western blot法检测各组细胞中Nrf2蛋白和Trx蛋白的相对表达量。

结果体外成功培养牛眼小梁细胞。不同浓度SFN干预下，细胞Trx mRNA相对表达量总体比较差异有统计学意义( F＝88.090， P<0.01)。LY294002＋SFN组、U0126＋SFN组和SFN组细胞中Trx蛋白和Nrf2蛋白相对表达量显著高于空白对照组，差异均有统计学意义(均 P<0.01)。LY294002＋SFN组和U0126＋SFN组细胞中Trx蛋白和Nrf2蛋白相对表达量显著高于SFN组，差异均有统计学意义(均 P<0.01)。

结论SFN可通过磷脂酰肌醇-3激酶(PI3K)/蛋白激酶B(Akt)通路与丝裂原活化蛋白激酶(MAPK)/细胞外调节蛋白激酶1/2(ERK1/2)通路活化Nrf2，促进小梁细胞Trx mRNA及其蛋白的合成，保护细胞抵抗氧化应激损伤。

莱菔硫烷;硫氧还蛋白;磷脂酰肌醇-3激酶;丝裂原活化蛋白激酶;Nrf2;小梁细胞
ABSTRACT

BackgroundRecent studies have confirmed that sulforaphane (SFN) can activate multiple pathways, and promote the expression of the antioxidants in cells.Thioredoxin (Trx) plays an important role in maintaining the intracellular redox in the steady state.

ObjectiveThis study was to investigate the effect and mechanism of SFN on Trx expression in bovine trabecular meshwork cells (BTMCs) cultured in vitro.

MethodsBTMCs were cultured in vitro and identified by morphological evaluation.The third generation of BTMCs were cultured in the medium with 0, 10, 20 and 30 μmol/L SFN for 30 minutes.Real-time PCR was applied to measure the expression of Trx mRNA in BTMCs.The BTMCs were randomly divided into normal control group, LY294002 group, U0126 group, SFN group, LY294002+ SFN group and U0126+ SFN group.The expressions of Nrf2 protein and Trx protein in each group were measured by Western blot.

ResultsThe BTMCs was successfully cultured in vitro.The expressions of Trx mRNA were significantly different among the different concentrationss of SFN treatment ( F=88.090, P<0.01). The expressions of Trx protein and Nrf2 protein in the LY294002+ SFN group, U0126+ SFN group and SFN group were significantly higher than those in the normal control group (all at P<0.01). The expressions of Trx protein and Nrf2 protein in the LY294002+ SFN group and U0126+ SFN group were significantly higher than those in the SFN group (all at P<0.01).

ConclusionsSFN can activate Nrf2 by phosphatidylinositol-3-kinase(PI3K)/protein kinase B (Akt) and mitogen-activated protein kinase (MAPK)/extracellular regulated protein kinases 1/2 (ERK1/2) signaling pathways, which can increase the expression level of Trx in BTMCs cultured in vitro.

Sulforaphane;Thioredoxin;Phosphotidylinositol-3-kinase;Mitogen-activated protein kinase;Nrf2;Trabecular meshwork cell
Wang Qiang, Email: mocdef.6ab21100qwyb
Fund program: Shandong Provincial Science and Technology Development Plan Policy Guidance Class (2012YD18117)
Copyright by Chinese Medical Association
No content published by the journals of Chinese Medical Association may be reproduced or abridged without authorization. Please do not use or copy the layout and design of the journals without permission.
All articles published represent the opinions of the authors, and do not reflect the official policy of the Chinese Medical Association or the Editorial Board, unless this is clearly specified.
引用本文

苏静,王强,刘玉震. 莱菔硫烷对体外培养牛眼小梁细胞硫氧还蛋白表达的影响及其机制[J]. 中华实验眼科杂志,2016,34(6)：504-508.

DOI:10.3760/cma.j.issn.2095-0160.2016.06.006

PERMISSIONS

Request permissions for this article from CCC.

评价本文
*以上评分为匿名评价

版权归中华医学会所有。

未经授权，不得转载、摘编本刊文章，不得使用本刊的版式设计。

除非特别声明，本刊刊出的所有文章不代表中华医学会和本刊编委会的观点。

原发性开角型青光眼(primary open angle glaucoma，POAG)是眼科常见的致盲疾病。近年的研究表明，氧化应激是小梁网损伤、眼压升高继而致盲的重要因素之一 [ 1 ]。硫氧还蛋白(thioredoxin，Trx)是一种由Nrf2介导的抗氧化蛋白，对维持细胞内氧化还原稳态起着重要作用 [ 2 , 3 ]，但Nrf2转录因子和Trx是否能够通过抗氧化应激作用对POAG进行预防和治疗及其机制尚不清楚。近期研究表明，莱菔硫烷(sulforaphane，SFN)可以在生物体中发挥抗炎以及抗氧化应激损伤的作用以维持内环境稳态。作为一种多功能的诱导物，SFN可通过激活Nrf2/ARE介导的多条促抗氧化蛋白表达的通路，发挥其维持机体细胞的氧化还原稳态、抗氧化应激损伤的功能。本研究中观察SFN对体外培养牛眼小梁细胞Trx表达的影响及其作用机制。
试读结束，您可以通过登录机构账户或个人账户后获取全文阅读权限。
参考文献
[1]
Caballero M , Liton PB , Challa P ,et al. Effects of donor age on proteasome activity and senescence in trabecular meshwork cells[J]. Biochem Biophys Res Commun, 2004,323(3):1048-1054. DOI: 10.1016/j.bbrc.2004.08.195 .
返回引文位置Google Scholar
百度学术
万方数据
[2]
李航，段惠军．Nrf2/ARE信号通路及其调控的抗氧化蛋白[J]．中国药理学通报， 2011，27(3)：300-303.
返回引文位置Google Scholar
百度学术
万方数据
Li H , Duan HJ . Nrf2/ARE pathway and downstream antioxidant genes[J]. Chin Pharmacological Bulletin, 2011,27(3):300-303.
Goto CitationGoogle Scholar
Baidu Scholar
Wanfang Data
[3]
Tanito M , Masutani H , Kim YC ,et al. Sulforaphane induces thioredoxin through the antioxidant-responsive element and attenuates retinal light damage in mice[J]. Invest Ophthalmol Vis Sci, 2005,46(3):979-987. DOI: 10.1167/iovs.04-1120 .
返回引文位置Google Scholar
百度学术
万方数据
[4]
张强，王强．肿瘤坏死因子-α对体外培养牛眼小梁细胞纤维连接蛋白的影响[J]．眼科新进展， 2009，29(11)：811-814.
返回引文位置Google Scholar
百度学术
万方数据
Zhang Q , Wang Q . Effects of tumor necrosis factor-α on fibronectin of cultured bovine trabecular meshwork cells in vitro[J]. Rec Adv Ophthalmol, 2009,29(11):811-814.
Goto CitationGoogle Scholar
Baidu Scholar
Wanfang Data
[5]
Porter KM , Epstein DL , Liton PB . Up-regulated expression of extracellular matrix remodeling genes in phagocytically challenged trabecular meshwork cells[J/OL]. PLoS One, 2012,7(4):e34792[2016-01-10]. http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3329506/. DOI: 10.1371/journal.pone.0034792 .
返回引文位置Google Scholar
百度学术
万方数据
[6]
Porter K , Hirt J , Stamer WD ,et al. Autophagic dysregulation in glaucomatous trabecular meshwork cells[J]. Biochim Biophys Acta, 2015,1852(3):379-385. DOI: 10.1016/j.bbadis.2014.11.021 .
返回引文位置Google Scholar
百度学术
万方数据
[7]
Zhao X , Zhang Y , Li X ,et al. Variations of thioredoxin system contributes to increased susceptibility to apoptosis in cardiomyocytes of type 2 diabetic rats[J]. Acta Biochim Biophys Sin (Shanghai), 2014,46(4):318-329. DOI: 10.1093/abbs/gmu006 .
返回引文位置Google Scholar
百度学术
万方数据
[8]
Shim YK , Kim JT , Seong MH ,et al. Serum thioredoxin 1 level has close relation with myocardial damage amount in acute myocardial infarction patients[J]. J Korean Med Sci, 2012,27(10):1162-1169. DOI: 10.3346/jkms.2012.27.10.1162 .
返回引文位置Google Scholar
百度学术
万方数据
[9]
黄蜜，戴爱国，蒋永亮．硫氧还蛋白与肺部疾病的研究进展[J]．临床与病理杂志， 2014，34(5)：606-610. DOI: 10.11714/j.issn.2095-6959.2014.05.030 .
返回引文位置Google Scholar
百度学术
万方数据
Huang M , Dai AG , Jiang YL . Recent progress of thioredoxin and lung-related diseases[J]. J Clin Pathol Res, 2014,34(5):606-610. DOI: 10.11714/j.issn.2095-6959.2014.05.030 .
Goto CitationGoogle Scholar
Baidu Scholar
Wanfang Data
[10]
张敏，王强，王康．糖基化终末产物对体外培养牛眼小梁细胞氧化应激及凋亡的影响[J]．眼科新进展， 2014，34(7)：640-642，646. DOI: 10.13389/j.cnki.rao.2014.0175 .
返回引文位置Google Scholar
百度学术
万方数据
Zhang M , Wang Q , Wang K . Effects of advanced glycation end products on oxidative stress and apoptosis of bovine trabecular meshwork cells cultured in vitro[J]. Rec Adv Ophthalmol, 2014,34(7):640-642, 646. DOI: 10.13389/j.cnki.rao.2014.0175 .
Goto CitationGoogle Scholar
Baidu Scholar
Wanfang Data
[11]
薛蔚，杜蜀华，李勇，等．压力对牛眼小梁细胞诱导型一氧化氮合酶mRNA的表达及蛋白质合成的影响[J]．中华眼科杂志， 2000，36(4)：295-298.
返回引文位置Google Scholar
百度学术
万方数据
Xue W , Du SH , Li Y ,et al. Pressure influence on mRNA expression and protein synthesis of inducible nitric oxide synthetase in bovine trabecular meshwork cell[J]. Chin J Ophthalmol, 2000,36(4):295-298.
Goto CitationGoogle Scholar
Baidu Scholar
Wanfang Data
[12]
Awai-Kasaoka N , Inoue T , Kameda T ,et al. Oxidative stress response signaling pathways in trabecular meshwork cells and their effects on cell viability[J]. Mol Vis, 2013,19:1332-1340.
返回引文位置Google Scholar
百度学术
万方数据
[13]
边江，曲明俐，王瑶，等．角膜基质细胞Nrf2-ARE信号通路活化缺陷在圆锥角膜发病中的作用[J]．中华实验眼科杂志， 2015，33(2)：109-114. DOI: 10.3760/cma.j.issn.2095-0160.2015.02.003 .
返回引文位置Google Scholar
百度学术
万方数据
Bian J , Qu ML , Wang Y ,et al. Defect of Nrf2-ARE signaling activation in corneal stromal cells of keratoconus[J]. Chin J Exp Ophthalmol, 2015,33(2):109-114. DOI: 10.3760/cma.j.issn.2095-0160.2015.02.003 .
Goto CitationGoogle Scholar
Baidu Scholar
Wanfang Data
[14]
Tkachev VO , Menshchikova EB , Zenkov NK . Mechanism of the Nrf2/Keap1/ARE signaling system[J]. Biochemistry (Mosc), 2011,76(4):407-422.
返回引文位置Google Scholar
百度学术
万方数据
[15]
Niture SK , Kaspar JW , Shen J ,et al. Nrf2 signaling and cell survival[J]. Toxicol Appl Pharmacol, 2010,244(1):37-42. DOI: 10.1016/j.taap.2009.06.009 .
返回引文位置Google Scholar
百度学术
万方数据
[16]
Tanito M , Masutani H , Kim YC ,et al. Sulforaphane induces thioredoxin through the antioxidant-responsive element and attenuates retinal light damage in mice[J]. Invest Ophthalmol Vis Sci, 2005,46(3):979-987. DOI: 10.1167/iovs.04-1120 .
返回引文位置Google Scholar
百度学术
万方数据
备注信息
A
王强，Email： mocdef.6ab21100qwyb
B
山东省科技发展计划政策引导类项目 (2012YD18117)
评论 （0条）
注册
登录
时间排序
暂无评论，发表第一条评论抢沙发
最新推荐
更多
小檗碱对糖尿病小鼠全层皮肤缺损创面愈合的影响及其机制
郑力铭 等
中华烧伤与创面修复杂志 2023,39(11)
高糖对体外培养视网膜Müller细胞自噬及凋亡的影响
王立 等
中华眼视光学与视觉科学杂志 2022,24(01)
莱菔硫烷对胃癌干细胞的影响及其机制研究
麦聪 等
中华胃肠外科杂志 2017,20(05)
高镁对体外培养原代神经干细胞分化命运的影响及其机制
廖旺 等
国际医药卫生导报 2016,22(11)
MedAI助手（体验版）
文档即答
智问智答
机器翻译
回答内容由人工智能生成，我社无法保证其准确性和完整性，该生成内容不代表我们的态度或观点，仅供参考。
生成快照
文献快照

你好，我可以帮助您更好的了解本文，请向我提问您关注的问题。

0/2000

《中华医学会杂志社用户协议》 | 《隐私政策》

《SparkDesk 用户协议》 | 《SparkDesk 隐私政策》

网信算备340104764864601230055号 | 网信算备340104726288401230013号

技术支持：

历史对话
本文全部
还没有聊天记录
设置
模式
纯净模式沉浸模式
字号
小中大