抗核抗体HEp－2细胞间接免疫荧光模型及其结果报告方式国际共识解读

胡朝军; 周仁芳; 张蜀澜; 秦晓松; 武永康; 牛家峰; 杨再兴; 何敏; 王春燕; 罗静; 黄清水; 李晞; 张铁汉; 李永哲

doi:10.3760/cma.j.issn.1009-9158.2016.11.002

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中华检验医学杂志

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ENGLISH ABSTRACT

抗核抗体HEp－2细胞间接免疫荧光模型及其结果报告方式国际共识解读

作者及单位信息

出版日期： 2016 -11 -11 ·

DOI： 10.3760/cma.j.issn.1009-9158.2016.11.002

Interpretation of the international consensus on standardized indirect immunofluorescence nomenclature of antinuclear antibody HEp-2 cell patterns and reporting ANA results

Authors Info & Affiliations

Hu Chaojun

Department of Rheumatology and Clinical Immunology, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences & Peking Union Medical College, Key Laboratory of Rheumatology and Clinical Immunology, Ministry of Education, Beijing 100032, China

Zhou Renfang

Zhang Shulan

Qin Xiaosong

Wu Yongkang

Niu Jiafeng

Yang Zaixing

He Min

Wang Chunyan

Luo Jing

Huang Qingshui

Li Xi

Zhang Tiehan

Li Yongzhe

Published： 2016 -11 -11 ·

DOI： 10.3760/cma.j.issn.1009-9158.2016.11.002

3624

367

APP内阅读

摘要

抗核抗体(ANA)是多种自身免疫病(AID)特别是ANA相关的风湿性疾病(AARD)诊断的重要血清标志物。以HEp－2细胞为基质的间接免疫荧光(IIF)法是ANA检测的重要方法，被美国风湿病学会(ACR)、欧洲自身免疫标准化促进会(EASI)等专业学会推荐为ANA检测的参考方法。2014年8月28日在巴西圣保罗举行的ANA荧光模型国际共识第一次会议上，来自15个国家的66位专家研讨形成ANA HEp－2细胞间接免疫荧光染色模型命名标准化国际共识。2015年9月22日在德国德累斯顿召开的ANA荧光模型国际共识第二次会议上形成了ANA结果报告方式国际共识。认真解读和推广ANA HEp－2细胞间接免疫荧光模型及其结果报告标准化国际共识有助于提高我国ANA检测质量和充分发挥ANA在AID临床诊疗中的价值。(中华检验医学杂志，2016, 39：804－810)

关键词：抗体，抗核;细胞系;荧光抗体技术，间接;模型，生物学

ABSTRACT

Antinuclear antibodies (ANA) are important serum markers in the diagnosis of a variety of autoimmune diseases(AID), especially ANA-associated rheumatic diseases (AARD). Indirect immunofluorescence assay using HEp-2 cells as substrate is an important method for the detection of ANA, which is considered as the reference method for ANA detection by the American College of Rheumatology (ACR) and the European Autoimmunity Standardization Initiative (EASI). The first meeting for the International Consensus on Antinuclear antibody Pattern (ICAP) was held in Sao Paulo, Brazil, on August 28, 2014. During the conference, a consensus on the nomenclature of staining patterns observed in the antinuclear antibody (ANA) indirect immunofluorescence test on HEp-2 cells was established by 66 experts representing 15 countries. The second meeting for the ICAP was held in Dresden, Germany, on 22 September 2015, and achieved a consensus regarding the reporting ANA test results. It is helpful to improve the quality of ANA testing in China and give full play to the value of ANA in the clinical diagnosis and treatment of AID, by careful interpretation and promotion of the international consensus on standardized indirect immunofluorescence nomenclature of antinuclear antibody HEp-2 cell patterns and reporting ANA results.( Chin J Lab Med, 2016, 39: 804-810)

KEYWORDS： Antibodies, antinuclear;Cell line;Fluorescent antibody technique, indirect;Models, biological

Corresponding author: Li Yongzhe, Email: mocdef.6ab21hcmupilehzgnoy

FUNDING:

National Natural Science Foundation of China (81172857, 81302610); the Capital Health Research and Development of Special Grants (2014-1-4011); Youth Research Foundation of Peking Union Medical College (3332015091)

COPYRIGHTS:

No content published by the journals of Chinese Medical Association may be reproduced or abridged without authorization. Please do not use or copy the layout and design of the journals without permission.

All articles published represent the opinions of the authors, and do not reflect the official policy of the Chinese Medical Association or the Editorial Board, unless this is clearly specified.

引用本文

胡朝军,周仁芳,张蜀澜,等. 抗核抗体HEp－2细胞间接免疫荧光模型及其结果报告方式国际共识解读[J]. 中华检验医学杂志,2016,39(11)：804-810.

DOI:10.3760/cma.j.issn.1009-9158.2016.11.002

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抗核抗体(antinuclear antibodies，ANA)是自身免疫病(autoimmune diseases，AID)重要的血清自身抗体，ANA的检测结果对AID的诊疗及病情评估具有重要作用。ANA是以真核细胞各种成分为靶抗原的非器官特异性自身抗体。自1957年采用间接免疫荧光(indirect immunofluorescence，IIF)法检测ANA以来 ^{[
1
]}，ANA的概念已经使用50余年。随着免疫荧光抗体技术的改进及人源培养细胞抗原基质的广泛应用，目前对ANA靶抗原的理解已由传统的细胞核成分扩展到包括细胞核、细胞浆、细胞骨架、细胞分裂周期蛋白等 ^{[
2
,

3
]}的整个细胞。ANA常用的检测方法包括使用纯化或重组抗原建立的ANA筛查方法，如ELISA和以HEp－2细胞为基质的IIF法等。前者具有操作简单、可实现自动化等优点，但是使用纯化或重组抗原检测ANA总抗体，存在纯化抗原过程中的抗原性"减弱"或"失活"，或重组抗原的决定簇缺乏部分天然的高级结构及无法完全重组全部抗原成分而导致假阴性等缺点。后者由于HEp－2细胞具有人源性、核抗原种类丰富、特异性强、含量高、核大、细胞结构清晰、易于结果观察及荧光染色模型分析，以及可以检测ANA总抗体，因此以HEp－2细胞为基质的IIF法仍然被美国风湿病学会(American College of Rheumatology，ACR)、欧洲自身免疫标准化促进会(European Autoimmunity Standardization Initiative，EASI)等专业学会推荐为ANA检测的"参考方法"。

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摘要
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备注信息

通信作者：李永哲，电子信箱： mocdef.6ab21hcmupilehzgnoy

基金项目：

国家自然科学基金 (81172857，81302610) 查看更多基金信息

首都卫生发展科研专项项目 (2014－1－4011) 查看更多基金信息

北京协和医学院协和青年基金 (3332015091) 查看更多基金信息

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