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原代人呼吸道上皮细胞培养体系(HAE)及其在病毒学研究中的应用
朱娜
谭文杰
作者及单位信息
·
DOI： 10.3760/cma.j.issn.0254-5101.2017.04.013
Primary human airway epithelial cell cultures: an update on technique and application in virology
Zhu Na
Tan Wenjie
Authors Info & Affiliations
Zhu Na
Key Laboratory for Medical Virology, National Health and Family Planning Commission, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China
Tan Wenjie
·
DOI： 10.3760/cma.j.issn.0254-5101.2017.04.013
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摘要

分化良好的原代人呼吸道上皮细胞培养体系(HAE)，是新近发展起来的呼吸道上皮组织体外重构技术。目前，该技术在国际上被广泛应用于病毒学的研究。本文将聚焦于HAE体系及其在呼吸道病毒研究中的应用，从呼吸道上皮的生理结构、固有免疫系统、体外重构方法以及HAE在呼吸道病毒研究中的应用等几个方面进行简要介绍以及系统的总结及展望。

原代人呼吸道上皮;呼吸道病毒;组织体外重构技术
ABSTRACT

Well-differentiated primary human airway epithelial cell culture (HAE), an organotypic human airway culture system, has been increasingly used as a linkage between researches conducted on animal models and humans. In this review, we focus on progress in technological development and its application in virology. The following aspects are covered in this review: (1)Structure and function of the normal airway, (2)Role of respiratory epithelial cells in pulmonary innate immunity, (3)Development of primary human airway epithelial cell cultures, (4)Application of primary human airway epithelial cell cultures in virology.

Primary human airway epithelial cell;Respiratory virus;Organotypic culture system
Tan Wenjie, Email: mocdef.3ab6182jwnat
National Natural Science Foundation of China (81301430); Major Project for Infectious Diseases (2014ZX10004-001, 2014ZX10004-002); National Key Research Program of China (2016YFD0500301)
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引用本文

朱娜,谭文杰. 原代人呼吸道上皮细胞培养体系(HAE)及其在病毒学研究中的应用[J]. 中华微生物学和免疫学杂志,2017,37(4)：309-314.

DOI:10.3760/cma.j.issn.0254-5101.2017.04.013

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分化良好的原代人呼吸道上皮细胞培养体系(well-differentiated human airway epithelial cell culture, HAE)，是新近发展起来的呼吸道上皮组织体外重构技术。该技术通过气-液两项的培养方式，使原代的人呼吸道上皮细胞在体外分化出呼吸道假复层纤毛柱状上皮的结构，并具备上皮分泌和转运功能。HAE良好地还原了呼吸道上皮在体内的结构和功能，同时也高度模拟了各种呼吸道病毒生长复制的微环境，因此该技术在国际上被广泛应用于病毒学的研究。本文将聚焦于HAE体系及其在呼吸道病毒研究中的应用，从呼吸道上皮的生理结构、固有免疫系统、体外重构方法、HAE在呼吸道病毒研究中的应用4个方面，简要介绍并系统总结了HAE在呼吸道病毒研究中的应用及展望。
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备注信息
A
谭文杰，Email: mocdef.3ab6182jwnat，电话：010-58900878
B
国家自然科学基金 (81301430)
传染病重大专项课题 (2014ZX10004-001，-002)
国家重点研发计划项目 (2016YFD0500301)
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