Development of a mouse model of stellate ganglion block and subsequent effects on cerebral cortical blood flow
Wang Jiahua, Zhou Wei, Wang Xiaohong, Yan Shiting, Tian Shunping, Wang Ying, Yu Le-yang, Li Hu, Zhang Dongsheng, Zhang Zhuan, Liu Weili
Abstract
ObjectiveTo develop a model of stellate ganglion block (SGB) in mice and investigate the effect of SGB on cerebral cortical blood flow.
MethodsThirty clean-grade healthy male C57BL/6 mice, aged 8-9 weeks, weighing 23-27 g, were divided into 5 groups (n=6 each) using a random number table method: control group (group C), left SGB group (group L), left normal saline group (group SL), right SGB group (group R) and right normal saline group (group SR). Group C received no intervention.SGB was performed with 0.25% ropivacaine 0.08 ml via percutaneous posterior approach in L and R groups, while the equal volume of normal saline 0.08 ml was given instead at the location of left and right stellate ganglion in SL and SR groups, respectively.The cerebral cortical blood flow was determined using laser speckle contrast imaging system before SGB (T0) and at 10, 30, 60, 90 and 120 min after SGB (T1-5).
ResultsMice developed ptosis on the block side, indicating that the model of SGB was successfully developed in L and R groups.There was no significant difference in cerebral cortical blood flow at each time point among C, SL and SR groups (P>0.05), and cerebral cortical blood flow on the block side decreased at T1, began to increase at T2, peaked at T3, and decreased at T5 which was still higher than that at T0 in group L and group R (P<0.01). Compared with C and SL groups, the left cerebral cortical blood flow was significantly decreased at T1, 5 and increased at T2-4 in group L (P<0.01). Compared with C and SR groups, the right cerebral cortical blood flow was significantly decreased at T1, 5 and increased at T2-4 in group R (P<0.01). There were no significant differences in cerebral cortical blood flow at each time point between group C and group SL and between group C and group RL (P>0.05).
ConclusionsThe mouse model of SGB via percutaneous posterior approach is successfully developed.Unilateral SGB can affect cerebral cortical blood flow on the block side, which shows a transitory decrease followed by a sustained significant increase.
Key words:
Stellate ganglion; Nerve block; Models, animal; Cerebrovascular circulation; Cerebral cortex
Contributor Information
Wang Jiahua
Department of Anesthesiology, the Affiliated Hospital of Yangzhou University, Yangzhou 225012, China
School of Medicine, Yangzhou University, Yangzhou 225009, China
Zhou Wei
Department of Anesthesiology, the Affiliated Hospital of Yangzhou University, Yangzhou 225012, China
Wang Xiaohong
School of Medicine, Yangzhou University, Yangzhou 225009, China
Yan Shiting
Department of Anesthesiology, the Affiliated Hospital of Yangzhou University, Yangzhou 225012, China
Graduate School, Dalian Medical University, Dalian 116000, China
Tian Shunping
Department of Anesthesiology, the Affiliated Hospital of Yangzhou University, Yangzhou 225012, China
Wang Ying
Department of Anesthesiology, the Affiliated Hospital of Yangzhou University, Yangzhou 225012, China
Graduate School, Dalian Medical University, Dalian 116000, China
Yu Le-yang
Department of Anesthesiology, the Affiliated Hospital of Yangzhou University, Yangzhou 225012, China
Graduate School, Dalian Medical University, Dalian 116000, China
Li Hu
Department of Anesthesiology, the Affiliated Hospital of Yangzhou University, Yangzhou 225012, China
Graduate School, Dalian Medical University, Dalian 116000, China
Zhang Dongsheng
Department of Anesthesiology, the Affiliated Hospital of Yangzhou University, Yangzhou 225012, China
Zhang Zhuan
Department of Anesthesiology, the Affiliated Hospital of Yangzhou University, Yangzhou 225012, China
Liu Weili
Department of Intensive Care, the Affiliated Hospital of Yangzhou University, Yangzhou 225012, China